BRENDA - Enzyme Database show
show all sequences of 1.14.13.183

Production of the dammarene sapogenin (protopanaxadiol) in transgenic tobacco plants and cultured cells by heterologous expression of PgDDS and CYP716A47

Chun, J.H.; Adhikari, P.B.; Park, S.B.; Han, J.Y.; Choi, Y.E.; Plant Cell Rep. 34, 1551-1560 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A47, recombinant expression under control of 35S CaMV promoter in leaves of tobacco via Agrobacterium tumefaciens GV3101 transformation method, RT-PCR expression analysis
Panax ginseng
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Panax ginseng
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dammarenediol-II + NADPH + H+ + O2
Panax ginseng
-
protopanaxadiol + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
H2DH16
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Panax ginseng
-
root
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dammarenediol-II + NADPH + H+ + O2
-
746053
Panax ginseng
protopanaxadiol + NADP+ + H2O
-
-
-
?
dammarenediol-II + NADPH + H+ + O2
hydroxylation of dammarenediol-II at C-12
746053
Panax ginseng
protopanaxadiol + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Panax ginseng
NADPH
-
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A47, recombinant expression under control of 35S CaMV promoter in leaves of tobacco via Agrobacterium tumefaciens GV3101 transformation method, RT-PCR expression analysis
Panax ginseng
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Panax ginseng
NADPH
-
Panax ginseng
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Panax ginseng
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dammarenediol-II + NADPH + H+ + O2
Panax ginseng
-
protopanaxadiol + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Panax ginseng
-
root
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dammarenediol-II + NADPH + H+ + O2
-
746053
Panax ginseng
protopanaxadiol + NADP+ + H2O
-
-
-
?
dammarenediol-II + NADPH + H+ + O2
hydroxylation of dammarenediol-II at C-12
746053
Panax ginseng
protopanaxadiol + NADP+ + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Panax ginseng
auxin treatment, particularly 2,4-dichlorophenoxyacetic acid, strongly enhances the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes and thus the production of dammarenediol-II and protopanaxadiol (PPD)
additional information
General Information
General Information
Commentary
Organism
metabolism
the enzyme is part of the biosynthetic pathway for ginsenoside production in Panax ginseng, overview
Panax ginseng
physiological function
protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has a wide range of pharmacological activities and high medicinal values. It is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is part of the biosynthetic pathway for ginsenoside production in Panax ginseng, overview
Panax ginseng
physiological function
protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has a wide range of pharmacological activities and high medicinal values. It is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme
Panax ginseng
Expression (protein specific)
Organism
Commentary
Expression
Panax ginseng
auxin treatment, particularly 2,4-dichlorophenoxyacetic acid, strongly enhances the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes and thus the production of dammarenediol-II and protopanaxadiol (PPD)
additional information
Other publictions for EC 1.14.13.183
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744549
Zhao
Optimization of a cytochrome ...
Panax ginseng
Biotechnol. Bioeng.
113
1787-1795
2016
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746053
Chun
Production of the dammarene s ...
Panax ginseng
Plant Cell Rep.
34
1551-1560
2015
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1
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745011
Wang
The isolation and characteriz ...
Panax quinquefolius
Funct. Integr. Genomics
14
545-557
2014
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