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Literature summary for 1.14.13.182 extracted from

  • Ritzmann, N.; Drees, S.; Fetznera, S.
    Signal synthase-type versus catabolic monooxygenases retracing 3-hydroxylation of 2-alkylquinolones and their N-oxides by Pseudomonas aeruginosa and other pulmonary pathogens (2021), Appl. Environ. Microbiol., 87, e02241 .
    View publication on PubMed
Search for more literature for 1.14.13.182

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa
expression in Escherichia coli Mycobacteroides abscessus
expression in Escherichia coli Microbulbifer sp. HZ11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00071
-
 2-heptylquinolin-4(1H)-one pH 7.8, 30°C Microbulbifer sp. HZ11
0.0975
-
 NADH pH 7.8, 30°C Microbulbifer sp. HZ11

Organism

Organism UniProt Comment Textmining
Microbulbifer sp. HZ11
-
-
-
Mycobacteroides abscessus B1MFK1
-
-
Mycobacteroides abscessus DSM 44196 B1MFK1
-
-
Pseudomonas aeruginosa Q9I0Q0
-
-
Pseudomonas aeruginosa DSM 22644 Q9I0Q0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-heptyl-1-hydroxyquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus 2-heptyl-1,3-dihydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-1-hydroxyquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus DSM 44196 2-heptyl-1,3-dihydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-1-hydroxyquinolin-4(1H)-one one + NADH + H+ + O2
-
 Pseudomonas aeruginosa 2-heptyl-1,3-dihydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-1-hydroxyquinolin-4(1H)-one one + NADH + H+ + O2
-
 Microbulbifer sp. HZ11 2-heptyl-1,3-dihydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-1-hydroxyquinolin-4(1H)-one one + NADH + H+ + O2
-
 Pseudomonas aeruginosa DSM 22644 2-heptyl-1,3-dihydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptylquinolin-4(1H)-one + NADH + H+ + O2 preferred substrate Mycobacteroides abscessus 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Pseudomonas aeruginosa 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Microbulbifer sp. HZ11 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptylquinolin-4(1H)-one + NADH + H+ + O2 preferred substrate Mycobacteroides abscessus DSM 44196 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Pseudomonas aeruginosa DSM 22644 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-nonylquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-nonylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Pseudomonas aeruginosa 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-nonylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Microbulbifer sp. HZ11 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-nonylquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus DSM 44196 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-nonylquinolin-4(1H)-one + NADH + H+ + O2 2-heptylquinolin-4(1H)-one and 2-nonylquinolin-4(1H)-one, preferred substrates Pseudomonas aeruginosa DSM 22644 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentylquinolin-4(1H)-one + NADH + H+ + O2
-
 Pseudomonas aeruginosa 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentylquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentylquinolin-4(1H)-one + NADH + H+ + O2
-
 Microbulbifer sp. HZ11 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentylquinolin-4(1H)-one + NADH + H+ + O2
-
 Mycobacteroides abscessus DSM 44196 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentylquinolin-4(1H)-one + NADH + H+ + O2
-
 Pseudomonas aeruginosa DSM 22644 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Aqm
-
 Mycobacteroides abscessus
PqsH
-
 Pseudomonas aeruginosa
PqsH
-
 Microbulbifer sp. HZ11

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 2-heptylquinolin-4(1H)-one pH 7.8, 30°C Microbulbifer sp. HZ11
0.3
-
 NADH pH 7.8, 30°C Microbulbifer sp. HZ11

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 NADH pH 7.8, 30°C Microbulbifer sp. HZ11
290
-
 2-heptylquinolin-4(1H)-one pH 7.8, 30°C Microbulbifer sp. HZ11