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Literature summary for 1.14.13.131 extracted from

  • Hammers, D.S.; Donaghy, C.M.; Heiss, S.L.; Harris, L.M.; Gordon, J.M.; Stevens, J.W.; Murray, L.P.; Schwab, A.D.; Hester, B.C.; Culpepper, M.A.
    Identification and characterization of a DmoB flavin oxidoreductase from a putative two-component DMS C-monooxygenase (2020), ACS Omega, 5, 9830-9838 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis upon expression in Escherichia coli, DmoA and DmoB subunits are able to generate product, albeit at a lower turnover than the natively expressed enzyme. No static protein-protein interactions are observed under the conditions tested between the two subunits Arthrobacter globiformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 NADH pH 7.4, 25°C Arthrobacter globiformis
0.018
-
 FMN pH 7.4, 25°C Arthrobacter globiformis
0.084
-
 FAD pH 7.4, 25°C Arthrobacter globiformis
0.142
-
 riboflavin pH 7.4, 25°C Arthrobacter globiformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 recombinant subunit DmoB, gel filtration Arthrobacter globiformis

Organism

Organism UniProt Comment Textmining
Arthrobacter globiformis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+ reaction of NAD(P)H-dependent flavin oxidoreductase subunit DmoB Arthrobacter globiformis FADH2 + NAD+
-
 ?
FMN + NADPH + H+ reaction of NAD(P)H-dependent flavin oxidoreductase subunit DmoB Arthrobacter globiformis FMN2 + NADP+
-
 ?
riboflavin + NADH + H+ reaction of NAD(P)H-dependent flavin oxidoreductase subunit DmoB Arthrobacter globiformis reduced riboflavin + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer recombinant subunit DmoB, 2 * 19000, SDS-PAGE, 2 * 19385, calculated from sequence Arthrobacter globiformis

Cofactor

Cofactor Comment Organism Structure
FMN NAD(P)H-dependent flavin oxidoreductase subunit DmoB prefers the cosubstrates FMN and NADH. binds at a 1:1 stoichiometry with Kd value 1.11 microM Arthrobacter globiformis
NADH NAD(P)H-dependent flavin oxidoreductase subunit DmoB prefers the cosubstrates FMN and NADH Arthrobacter globiformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0003
-
 riboflavin pH 7.4, 25°C Arthrobacter globiformis
0.0006
-
 FAD pH 7.4, 25°C Arthrobacter globiformis
0.004
-
 FMN pH 7.4, 25°C Arthrobacter globiformis
0.021
-
 NADH pH 7.4, 25°C Arthrobacter globiformis