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Literature summary for 1.14.13.131 extracted from

  • Boden, R.; Borodina, E.; Wood, A.; Kelly, D.; Murrell, J.; Schäfer, H.
    Purification and characterization of dimethylsulfide monooxygenase from Hyphomicrobium sulfonivorans (2011), J. Bacteriol., 193, 1250-1258.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.131

Cloned(Commentary)

Cloned (Comment) Organism
53 kDa subunit DmoA Hyphomicrobium sulfonivorans

Inhibitors

Inhibitors Comment Organism Structure
8-anilinonaphthalenesulfonate 1 mM, almost complete inhibition Hyphomicrobium sulfonivorans
Cd2+ 1 mM, about 15% residual activity Hyphomicrobium sulfonivorans
Hg2+ 1 mM, about 5% residual activity Hyphomicrobium sulfonivorans
Pb2+ 1 mM, about 10% residual activity Hyphomicrobium sulfonivorans
umbelliferone 1 mM, almost complete inhibition Hyphomicrobium sulfonivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0172
-
 Dimethyl sulfide pH 7.4, 30°C Hyphomicrobium sulfonivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 18% restauration of activity in preparations previously depleted of cations, Mg2+ and Fe2+ together lead to 63% restauration Hyphomicrobium sulfonivorans
Mg2+ 10% restauration of activity in preparations previously depleted of cations, Mg2+ and Fe2+ together lead to 63% restauration Hyphomicrobium sulfonivorans
additional information divalent cation required, no residual monooxygenase activity in preparations previously depleted of cations. None of the divalent cations, added alone or in combination, fully restores activity Hyphomicrobium sulfonivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 1 * 53000 + 1 * 19000, SDS-PAGE. DmoA, 53-kDa FMNH2-dependent monooxygenase, and DmoB, 19-kDa NAD(P)H-dependent flavin oxidoreductase subunit Hyphomicrobium sulfonivorans
53000
-
 1 * 53000 + 1 * 19000, SDS-PAGE. DmoA, 53-kDa FMNH2-dependent monooxygenase, and DmoB, 19-kDa NAD(P)H-dependent flavin oxidoreductase subunit Hyphomicrobium sulfonivorans
73000
-
 gel filtration Hyphomicrobium sulfonivorans

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sulfonivorans E9JFX9 large subunit DmoA; enzyme belongs to flavin-linked monooxygenases of the luciferase family
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyphomicrobium sulfonivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.256
-
 pH 7.4, 30°C Hyphomicrobium sulfonivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethyl sulfide + O2 + NADH about 35% of the activity with dimethyl sulfide Hyphomicrobium sulfonivorans ethanethiol + acetaldehyde + NAD+ + H2O
-
 ?
dimethyl sulfide + O2 + NADH
-
 Hyphomicrobium sulfonivorans methanethiol + formaldehyde + NAD+ + H2O
-
 ?
ethylmethyl sulfide + O2 + NADH about 50% of the activity with dimethyl sulfide Hyphomicrobium sulfonivorans ? + NAD+ + H2O
-
 ?
additional information no activity with alkanesulfonates, aldehydes, nitrilotriacetate, or dibenzothiophenesulfone Hyphomicrobium sulfonivorans ?
-
 ?
propylmethyl sulfide + O2 + NADH about 35% of the activity with dimethyl sulfide Hyphomicrobium sulfonivorans ? + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 53000 + 1 * 19000, SDS-PAGE. DmoA, 53-kDa FMNH2-dependent monooxygenase, and DmoB, 19-kDa NAD(P)H-dependent flavin oxidoreductase subunit Hyphomicrobium sulfonivorans

Synonyms

Synonyms Comment Organism
dimethylsulfide monooxygenase
-
 Hyphomicrobium sulfonivorans
DMS monooxygenase
-
 Hyphomicrobium sulfonivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.45
-
 Dimethyl sulfide pH 7.4, 30°C Hyphomicrobium sulfonivorans

Cofactor

Cofactor Comment Organism Structure
FMN highest activity with cofactors FMN and NADH Hyphomicrobium sulfonivorans
additional information little or no activity is observed with FAD, lumiflavin, riboflavin, or lumichrome in the presence of NADPH Hyphomicrobium sulfonivorans
NADH highest activity with cofactors FMN and NADH. NADH plus FAD show 64% of the activity with NADH and FMN Hyphomicrobium sulfonivorans
NADPH plus FMN, 4% of the activity with cofactors FMN and NADH Hyphomicrobium sulfonivorans