Literature summary for 1.14.13.1 extracted from

Fang, Y.; Bullock, H.; Lee, S.A.; Sekar, N.; Eiteman, M.A.; Whitman, W.B.; Ramasamy, R.P.
Detection of methyl salicylate using bi-enzyme electrochemical sensor consisting salicylate hydroxylase and tyrosinase (2016), Biosens. Bioelectron., 85, 603-610 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL1-Blue cells	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
salicylate + NADH + H+ + O2	Pseudomonas putida	-	catechol + NAD+ + H2O + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	after 19fold purification, the enzyme shows a specific activity of 12.34 units/mg	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
salicylate + NADH + H+ + O2	-	Pseudomonas putida	catechol + NAD+ + H2O + CO2	-	?

Synonyms

Synonyms	Comment	Organism
NahG	-	Pseudomonas putida
salicylate hydroxylase	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas putida
NADH	-	Pseudomonas putida

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Release 2023.1 (January 2023)