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Literature summary for 1.14.12.18 extracted from

  • Barriault, D.; Plante, M.M.; Sylvestre, M.
    Family shuffling of a targeted bphA region to engineer biphenyl dioxygenase (2002), J. Bacteriol., 184, 3794-3800.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.12.18

Protein Variants

Protein Variants Comment Organism
N348H wild-type enzyme shows less than 10% activity with 2,6-dichlorobiphenyl, 4,4'-dichlorobiphenyl - mutant enzyme shows about 55% depletion. Wild-type enzyme shows no degradation of 2,4,4'-trichlorobiphenyl and 2,2',5,5'-tetrachlorobiphenyl - mutant enzyme shows 50% and 92% depletion Comamonas testosteroni
N348H/A404V wild-type enzyme shows less than 10% activity with 2,6-dichlorobiphenyl, 4,4'-dichlorobiphenyl - mutant enzyme shows about 55% depletion. Wild-type enzyme shows no degradation of 2,4,4'-trichlorobiphenyl and 2,2',5,5'-tetrachlorobiphenyl - mutant enzyme shows 29% and 84% depletion Comamonas testosteroni
T335G/F336I/N338T/I341T relaxation of the enzyme towards polychlorinated biphenyls. Wild-type enzyme shows less than 10% degradation with 2,6-dichlorobiphenyl, 3,3'-dichlorobiphenyl, 4,4'-dichlorobiphenyl, 2,3',4'-trichlorobiphenyl - the mutant enzyme enzyme shows 45 to 99% depletion depending on the substrate. Wild-type enzyme shows no degradation of 2,4,4'-trichlorobiphenyl, mutant enzyme shows 44% depletion Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-
Burkholderia sp. LB4000
-
-
-
Comamonas testosteroni
-
 B-356
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2',3,3'-tetrachlorobiphenyl + NADH + O2
-
 Comamonas testosteroni ?
-
 ?
2,2',3,3'-tetrachlorobiphenyl + NADH + O2
-
 Burkholderia sp. ?
-
 ?
2,2',3,3'-tetrachlorobiphenyl + NADH + O2
-
 Burkholderia sp. LB4000 ?
-
 ?
2,2',5,5'-tetrachlorobiphenyl + NADH + O2
-
 Burkholderia sp. ?
-
 ?
2,2',5,5'-tetrachlorobiphenyl + NADH + O2
-
 Burkholderia sp. LB4000 ?
-
 ?
2,3',4'-trichlorobiphenyl + NADH + O2
-
 Comamonas testosteroni ?
-
 ?
2,3,4'-trichlorobiphenyl + NADH + O2
-
 Comamonas testosteroni ?
-
 ?
3,3'-dichlorobiphenyl + NADH + O2
-
 Comamonas testosteroni ?
-
 ?

Synonyms

Synonyms Comment Organism
BPDO
-
 Comamonas testosteroni
BPDO
-
 Burkholderia sp.