BRENDA - Enzyme Database
show all sequences of 1.14.11.9

Overexpression of a tea flavanone 3-hydroxylase gene confers tolerance to salt stress and Alternaria solani in transgenic tobacco

Mahajan, M.; Yadav, S.K.; Plant Mol. Biol. 85, 551-573 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt
Camellia sinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis
-
dihydroquercetin + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydroquercetin + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Camellia sinensis
-
dihydrokaempferol + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Camellia sinensis
Q6DV45
from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-
Camellia sinensis UPASI-10
Q6DV45
from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydroquercetin + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydroquercetin + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydrokaempferol + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CsF3H
-
Camellia sinensis
flavanone 3-hydroxylase
-
Camellia sinensis
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt
Camellia sinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis
-
dihydroquercetin + succinate + CO2
-
-
?
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydroquercetin + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Camellia sinensis
-
dihydrokaempferol + succinate + CO2
-
-
?
naringenin + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydroquercetin + succinate + CO2
-
-
-
?
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydroquercetin + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydrokaempferol + succinate + CO2
-
-
-
?
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydrokaempferol + succinate + CO2
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis
Camellia sinensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis
Camellia sinensis
Other publictions for EC 1.14.11.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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J. Biotechnol.
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1
1
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1
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5
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2
2
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744729
Chaipanya
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Isolation and expression anal ...
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Chiang Mai J. Sci.
44
427-437
2017
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2
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2
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2
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2
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2
2
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745484
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Molecular cloning and functio ...
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2017
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1
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1
1
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745623
Khumkarjorn
-
Cloning and expression analys ...
x Ascocenda, x Ascocenda NK-2012
J. Plant Biochem. Biotechnol.
26
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2017
1
-
1
-
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1
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1
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4
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2
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4
1
1
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1
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1
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1
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746071
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PnF3H, a flavanone 3-hydroxyl ...
Pohlia nutans
Plant Growth Regul.
83
489-500
2017
1
-
1
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1
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1
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1
1
1
1
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744604
Tu
Molecular characterization of ...
Carthamus tinctorius
BMC Plant Biol.
16
132
2016
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1
-
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3
1
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1
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6
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1
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1
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1
1
2
2
1
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746122
Xiong
Molecular cloning and charact ...
Artemisia annua
Plant Physiol. Biochem.
105
29-36
2016
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-
1
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1
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2
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1
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1
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2
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1
1
1
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1
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1
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746138
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Molecular cloning and identif ...
Lycium chinense
Plant Physiol. Biochem.
98
89-100
2016
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1
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6
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2
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2
2
2
2
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745621
Kumar
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Functional characterization o ...
Phyllanthus emblica
J. Plant Biochem. Biotechnol.
24
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2015
1
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1
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1
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1
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3
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742549
Flores
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Fragaria x ananassa, Ribes nigrum, Ribes rubrum, Rubus idaeus, Rubus plicatus, Vaccinium myrtillus
Food Chem.
153
130-133
2014
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5
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8
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11
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13
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15
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5
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743524
Li
Flower color patterning in pa ...
Viola x wittrockiana
Plant Physiol. Biochem.
84
134-141
2014
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1
1
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743971
Zhang
-
Cloning and functional analys ...
Reaumuria trigyna
Acta Physiol. Plant.
36
1221-1229
2014
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1
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1
3
6
2
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746091
Mahajan
Overexpression of a tea flava ...
Camellia sinensis, Camellia sinensis UPASI-10
Plant Mol. Biol.
85
551-573
2014
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1
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6
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1
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6
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1
1
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726211
Liu
Regulation of flavanone 3-hydr ...
Hololachna songarica
Plant Physiol. Biochem.
73C
161-167
2013
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1
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726318
Cheng
Diversifying selection on flav ...
Glycine max, Glycine soja
PLoS ONE
8
e54154
2013
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2
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4
4
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726200
Flachowsky
Silencing of flavanone-3-hydro ...
Malus domestica
Plant Physiol. Biochem.
51
18-25
2012
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1
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1
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3
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1
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1
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2
2
1
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725165
Himi
Differential expression of thr ...
Triticum aestivum
Int. J. Plant Genomics
2011
369460
2011
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3
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10
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3
9
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713238
Lovdal
Synergetic effects of nitrogen ...
Solanum lycopersicum, Solanum lycopersicum Suzanne
Phytochemistry
71
605-613
2010
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1
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2
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1
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2
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1
1
1
1
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713256
Liu
Molecular dissection of the pa ...
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1173-1185
2010
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1
3
3
1
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-
698431
Halbwirth
Measuring flavonoid enzyme act ...
no activity in Actinidia deliciosa, Prunus avium, Prunus cerasus, Prunus domestica, Ribes uva-crispa, Rubus idaeus, Rubus plicatus, Sambucus nigra
J. Agric. Food Chem.
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7
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22
28
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7
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28
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21
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700130
Cheng
-
Polymorphisms of soybean isofl ...
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Mol. Breed.
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2009
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2
2
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2
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700225
Khlestkina
Anthocyanin biosynthesis genes ...
Secale cereale
Mol. Genet. Genomics
282
475-485
2009
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1
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4
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700742
Zheng
-
Sugars induce anthocyanin accu ...
Vitis vinifera
Plant Growth Regul.
58
251-260
2009
1
1
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-
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1
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1
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700794
Eungwanichayapant
Accumulation of catechins in t ...
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Plant Physiol. Biochem.
47
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2009
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1
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1
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688942
Kim
Accumulation of flavonols in r ...
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Mol. Cells
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2008
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4
1
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688943
Kim
Flavanone 3beta-hydroxylases f ...
Oryza sativa
Mol. Cell
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2008
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1
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1
3
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3
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5
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1
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12
1
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1
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3
3
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3
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3
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3
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3
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12
3
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689653
Owens
Biochemical and genetic charac ...
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2008
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1
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1
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1
-
1
-
1
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690168
Singh
An early gene of the flavonoid ...
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2008
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1
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1
1
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1
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Hukkanen
Benzothiadiazole affects the l ...
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701213
Nakatsuka
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Isolation and expression analy ...
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2008
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746540
Singh
An early gene of the flavonoi ...
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1
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1
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1
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1
1
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1
1
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689577
Gebhardt
Evolution of flavone synthase ...
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16
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Combinatorial biosynthesis of ...
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1
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674117
Halbwirth
Two-phase flavonoid formation ...
Fragaria x ananassa
J. Agric. Food Chem.
54
1479-1485
2006
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Leonard
Functional expression of a P45 ...
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2
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676671
Halbwirth
-
Screening for inhibitors of 2- ...
Malus domestica, Pyrus communis
Plant Sci.
171
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2
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12
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2
2
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22
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2
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2
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4
-
2
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2
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658733
Wellmann
Significance of C-terminal seq ...
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FEBS Lett.
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2004
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1
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1
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Divergent evolution of flavono ...
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660098
Pelt
Flavanone 3-hydroxylase expres ...
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64
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2003
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439125
Lukacin
Site-directed mutagenesis of t ...
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2000
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Lukacin
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Molecular characterization of ...
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Britsch
Purification and characterizat ...
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Purification of flavanone 3 be ...
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439123
O'Neill
Molecular genetic analysis of ...
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-
-
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-
-
-
-
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1
-
1
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-
-
-
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439115
Beerhues
-
Flavanone 3-hydroxylase and di ...
Tulipa hybrid cultivar
J. Plant Physiol.
133
743-746
1989
-
-
-
-
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439116
Takeda
-
Anthocyanin composition of Sin ...
Sinapis alba
Phytochemistry
27
1351-1353
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
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-
-
1
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-
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-
1
-
-
1
-
-
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-
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-
439119
Britsch
Purification and characterizat ...
Petunia x hybrida
Eur. J. Biochem.
156
569-577
1986
2
-
-
-
-
-
10
4
-
2
3
-
-
2
1
-
1
-
-
1
1
1
3
1
-
-
-
-
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
-
10
2
4
-
2
3
-
-
1
-
1
-
1
1
1
3
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439120
Forkmann
Selection and characterisation ...
Dahlia pinnata, Glandularia x hybrida, Streptocarpus hybrida, Zinnia elegans
Planta
161
261-265
1984
-
-
-
-
-
-
12
-
4
-
-
4
-
7
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
4
-
-
4
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438998
Dangelmayr
-
Relationship between flower de ...
Matthiola incana
Z. Naturforsch. C
38
551-555
1983
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-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
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-
-
4
-
-
2
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-
-
-
-
-
-
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-
-
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-
2
-
-
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-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439001
Forkmann
-
Genetic control of flavanone 3 ...
Antirrhinum majus
Z. Naturforsch. C
36
411-416
1981
-
-
-
-
-
-
-
-
1
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-
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-
1
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-
1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439006
Forkmann
-
Anthocyanin biosynthesis in fl ...
Matthiola incana
Z. Naturforsch. C
35
691-695
1980
1
-
-
-
-
-
5
-
1
1
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
1
1
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
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-
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-
-