Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Oscillatoria sp. PCC 7926 |
Protein Variants | Comment | Organism |
---|---|---|
D159A | inactive | Oscillatoria sp. PCC 7926 |
H157A | inactive | Oscillatoria sp. PCC 7926 |
H247A | inactive | Oscillatoria sp. PCC 7926 |
R257A | inactive | Oscillatoria sp. PCC 7926 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-deoxycylindrospermopsin | the enzyme is strongly inhibited by 7-deoxycylindrospermopsin at concentration higher than 0.001 mM | Oscillatoria sp. PCC 7926 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
2-oxoglutarate | pH 7.4 and 28°C | Oscillatoria sp. PCC 7926 | |
0.019 | - |
7-deoxycylindrospermopsin | pH 7.4 and 28°C | Oscillatoria sp. PCC 7926 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on. Km value is 0.0021 mM. 0.05 mM used in assay conditions | Oscillatoria sp. PCC 7926 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 | Oscillatoria sp. PCC 7926 | - |
7-epi-cylindrospermopsin + succinate + CO2 | - |
? | |
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 | Oscillatoria sp. PCC 7926 | - |
cylindrospermopsin + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oscillatoria sp. PCC 7926 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap nickel column chromatography and Superdex S200 gel filtration | Oscillatoria sp. PCC 7926 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 | - |
Oscillatoria sp. PCC 7926 | 7-epi-cylindrospermopsin + succinate + CO2 | - |
? | |
7-deoxycylindrospermopsin + 2-oxoglutarate + O2 | - |
Oscillatoria sp. PCC 7926 | cylindrospermopsin + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyrI | - |
Oscillatoria sp. PCC 7926 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
2-oxoglutarate | pH 7.4 and 28°C | Oscillatoria sp. PCC 7926 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.3 | - |
7-deoxycylindrospermopsin | pH 7.4 and 28°C | Oscillatoria sp. PCC 7926 |