Application | Comment | Organism |
---|---|---|
synthesis | an Escherichia coli cell factory expressing the Pseudomonas stutzeri ectD gene from a synthetic promoter imports homoectoine via the ProU and ProP compatible solute transporters, hydroxylates it, and secretes the formed trans-5-hydroxyhomoectoine, independent from all currently known mechanosensitive channels, into the growth medium | Stutzerimonas stutzeri |
Crystallization (Comment) | Organism |
---|---|
modeling and docking of different substrates into the crystal structure. The spatial positioning of homoectoine and its hydroxylated derivative in the reaction chamber are super-imposable with that of the 5-hydroxyectoine molecule | Sphingopyxis alaskensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
ectoine | pH 7.5, 35°C | Sphingopyxis alaskensis | |
2.3 | - |
ectoine | pH 7.5, 35°C | Stutzerimonas stutzeri | |
2.6 | - |
L-homoectoine | pH 7.5, 35°C | Stutzerimonas stutzeri | |
3.1 | - |
L-homoectoine | pH 7.5, 35°C | Sphingopyxis alaskensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis alaskensis | Q1GNW5 | - |
- |
Sphingopyxis alaskensis DSM 13593 | Q1GNW5 | - |
- |
Stutzerimonas stutzeri | A4VFY4 | i.e. Pseudomonas stutzeri | - |
Stutzerimonas stutzeri A1501 | A4VFY4 | i.e. Pseudomonas stutzeri | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis | 5-hydroxyectoine + succinate + CO2 | - |
? | |
ectoine + 2-oxoglutarate + O2 | - |
Stutzerimonas stutzeri | 5-hydroxyectoine + succinate + CO2 | - |
? | |
ectoine + 2-oxoglutarate + O2 | - |
Stutzerimonas stutzeri A1501 | 5-hydroxyectoine + succinate + CO2 | - |
? | |
ectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis DSM 13593 | 5-hydroxyectoine + succinate + CO2 | - |
? | |
L-homoectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis | trans-5-hydroxy-L-homoectoine + succinate + CO2 | - |
? | |
L-homoectoine + 2-oxoglutarate + O2 | - |
Stutzerimonas stutzeri | trans-5-hydroxy-L-homoectoine + succinate + CO2 | - |
? | |
L-homoectoine + 2-oxoglutarate + O2 | - |
Stutzerimonas stutzeri A1501 | trans-5-hydroxy-L-homoectoine + succinate + CO2 | - |
? | |
L-homoectoine + 2-oxoglutarate + O2 | - |
Sphingopyxis alaskensis DSM 13593 | trans-5-hydroxy-L-homoectoine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34140, calculated from sequence | Sphingopyxis alaskensis |
? | x * 34180, calculated from sequence and SDS-PAGE | Stutzerimonas stutzeri |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sphingopyxis alaskensis | calculated from sequence | - |
5.5 |
Stutzerimonas stutzeri | calculated from sequence | - |
5.5 |