Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum ssp. lactofermentum strain SN01 | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis | |
0.61 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | Bacillus thuringiensis YBT-1520 | - |
(4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
- |
- |
Bacillus thuringiensis YBT-1520 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus thuringiensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:corn steep liquor-grown cell | - |
Bacillus thuringiensis | - |
Storage Stability | Organism |
---|---|
25°C, purified enzyme, 1 h and 4 h, 18% and more than 70% loss of activity, respectively | Bacillus thuringiensis |
4°C, purified enzyme, 2 and 8 days, 30% and more than 85% loss of activity, respectively | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? | |
L-isoleucine + 2-oxoglutarate + O2 | - |
Bacillus thuringiensis YBT-1520 | (4S)-4-hydroxy-L-isoleucine + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Bacillus thuringiensis |
? | x * 29550, calculated from amino acid sequence | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Bacillus thuringiensis |
L-isoleucine dioxygenase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | preincubating the enzyme at 50°C for 1 h results in a loss of approximately half of activity, and nearly no activity is detected after preheating at 70°C for 1 h | Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis | |
0.089 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | about 25% activity at pH 5.5 and 7.0, respectively, about 50% activity at pH 6.5 | Bacillus thuringiensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | the enzyme remains stable between pH 5.0 and 8.0 for 24 h | Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
2-oxoglutarate | at pH 6.0 and 40°C | Bacillus thuringiensis | |
0.146 | - |
L-isoleucine | at pH 6.0 and 40°C | Bacillus thuringiensis |