Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.11.4 extracted from

  • Kogan, N.M.; Melamed, E.; Wasserman, E.; Raphael, B.; Breuer, A.; Stok, K.S.; Sondergaard, R.; Escudero, A.V.; Baraghithy, S.; Attar-Namdar, M.; Friedlander-Barenboim, S.; Mathavan, N.; Isaksson, H.; Mechoulam, R.; Mueller, R.; Bajayo, A.; Gabet, Y.; Bab, I.
    Cannabidiol, a major non-psychotropic cannabis constituent enhances fracture healing and stimulates lysyl hydroxylase activity in osteoblasts (2015), J. Bone Miner. Res., 30, 1905-1913 .
    View publication on PubMed
Search for more literature for 1.14.11.4

Activating Compound

Activating Compound Comment Organism Structure
cannabidiol CBD, a major non-psychotropic cannabis constituent, stimulates lysyl hydroxylase 1 activity in osteoblasts, cannabidiol enhances the biomechanical properties of healing rat mid-femoral fractures Rattus norvegicus
DELTA9-tetrahydrocannabinol THC, the psychotropic cannabis constituent, stimulates lysyl hydroxylase 2 activity in osteoblasts Rattus norvegicus
additional information no stimulating effect of LH1 by DELTA9-tetrahydrocannabinol (THC), but THC potentiates the CBD stimulated work-to-failure at 6 weeks postfracture followed by attenuation of the CBD effect at 8 weeks Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
gene Plod1, real-time RT-PCR enzyme expression analysis Rattus norvegicus
gene Plod2, real-time RT-PCR enzyme expression analysis Rattus norvegicus
gene Plod3, real-time RT-PCR enzyme expression analysis Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[procollagen]-L-lysine + 2-oxoglutarate + O2 Rattus norvegicus
-
 [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q5U367
-
-
Rattus norvegicus Q63321
-
-
Rattus norvegicus Q811A3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
 Rattus norvegicus
-
osteoblast
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[procollagen]-L-lysine + 2-oxoglutarate + O2
-
 Rattus norvegicus [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
 ?

Synonyms

Synonyms Comment Organism
LH1
-
 Rattus norvegicus
LH2
-
 Rattus norvegicus
LH3
-
 Rattus norvegicus
Plod1
-
 Rattus norvegicus
PLOD2
-
 Rattus norvegicus
PLOD3
-
 Rattus norvegicus

Expression

Organism Comment Expression
Rattus norvegicus no induction of PLOD3 expression in osteoblasts by DELTA9-tetrahydrocannabinol and cannabidiol additional information
Rattus norvegicus cannabidiol stimulates mRNA expression of Plod1 in primary osteoblast cultures up
Rattus norvegicus DELTA9-tetrahydrocannabinol stimulates mRNA expression of Plod2 in primary osteoblast cultures up

General Information

General Information Comment Organism
physiological function the enzyme catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. Critical mechanical role of collagen crosslinking enzymes, e.g. in bone healing. Lysyl hydroxylase 1 (PLOD1) is one of the few collagen crosslinking enzymes associated with bone quality Rattus norvegicus
physiological function the enzyme LH2 catalyzes lysine hydroxylation, which is involved in collagen crosslinking and stabilization. PLOD2 has no role in determining bone quality Rattus norvegicus
physiological function the enzyme LH3 catalyzes lysine hydroxylation Rattus norvegicus