Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required for activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene PLOD2, large-scale transfection with N-terminal His8 and human growth hormone (hGH) tags transfection with polyethylenimine and recombinant expression of wild-type enzyme, N-terminally truncated enzyme mutant, and of inactive mutant D689A in engineered CHO cells and secretion to the cell culture medium | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D689A | inactive mutant | Homo sapiens |
additional information | generation of an N-terminally truncated enzyme mutant comprising residues 33-758 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | competitive substrate inhibition | Homo sapiens | |
L-alpha-hydroxyglutarate | the enzyme activity is inhibited by L-alpha-hydroxyglutarate but not its enantiomer D-alpha-hydroxyglutarate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of LH2 | Homo sapiens | |
0.011 | - |
2-oxoglutarate | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.169 | - |
peptide (IKG)3 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[procollagen]-L-lysine + 2-oxoglutarate + O2 | Homo sapiens | - |
[procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00469 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | identification of LH2 glycosylation by nanoLC MS/MS analysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | an enzyme activity assay for detection of succinate is evaluated: the succinate-Glo assay, employing a luciferase-based luminescence system, is superior from the standpoint of stability and signal-to-noise ratio. Luciferase activity is detected over a wide range of concentrations of LH2, and the luminescence signal was proportional to the concentration of LH2. Method development and evaluation, overview | Homo sapiens | ? | - |
? | |
peptide (IKG)3 + 2-oxoglutarate + O2 | - |
Homo sapiens | ? | - |
? | |
[procollagen]-L-lysine + 2-oxoglutarate + O2 | - |
Homo sapiens | [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LH2 | - |
Homo sapiens |
lysyl hydroxylase 2 | - |
Homo sapiens |
PLOD2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.183 | - |
2-oxoglutarate | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.2 | - |
peptide (IKG)3 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the 2-oxoglutarate/Fe2+-dependent oxygenase family | Homo sapiens |
physiological function | hydroxylysine aldehyde-derived collagen cross-links (HLCCs) accumulate in fibrotic tissues and certain types of cancer and are thought to drive the progression of these diseases. HLCC formation is initiated by lysyl hydroxylase 2 (LH2), an Fe(II) and 2-oxoglutarate-dependent oxygenase that hydroxylates telopeptidyl lysine residues on collagen | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.18 | - |
peptide (IKG)3 | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
16.6 | - |
2-oxoglutarate | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens |