BRENDA - Enzyme Database show
show all sequences of 1.14.11.17

alpha-Hydroxylation of carboxylic acids catalyzed by taurine dioxygenase

Wetzl, D.; Bolsinger, J.; Nestl, B.; Hauer, B.; ChemCatChem 8, 1361-1366 (2016)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21(DE3)
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
F206Y
the conversions of the improved catalyst increases by at least 140% compared to that of the wild-type enzyme and shows broadened substrate scope
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P37610
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutyric acid + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 4.7fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-4-aminobutyric acid + succinate + CO2
-
-
-
?
5-aminovaleric acid + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 4.4fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-5-aminovaleric acid + succinate + CO2
-
-
-
?
6-aminocaproic acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-6-aminocaproic acid + succinate + CO2
-
-
-
?
alpha-methyl-beta-alanine + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
3-amino-2-hydroxy-2-methylpropanoic acid + succinate + CO2
-
-
-
?
beta-alanine + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 2.4fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-isoserine + succinate + CO2
-
-
-
?
butyric acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxy-4-butyric acid + succinate + CO2
-
-
-
?
propionic acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxypropionic acid + succinate + CO2
-
-
-
?
valeric acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxyvaleric acid + succinate + CO2
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21(DE3)
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F206Y
the conversions of the improved catalyst increases by at least 140% compared to that of the wild-type enzyme and shows broadened substrate scope
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-aminobutyric acid + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 4.7fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-4-aminobutyric acid + succinate + CO2
-
-
-
?
5-aminovaleric acid + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 4.4fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-5-aminovaleric acid + succinate + CO2
-
-
-
?
6-aminocaproic acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
D-2-hydroxy-6-aminocaproic acid + succinate + CO2
-
-
-
?
alpha-methyl-beta-alanine + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
3-amino-2-hydroxy-2-methylpropanoic acid + succinate + CO2
-
-
-
?
beta-alanine + 2-oxoglutarate + O2
activity with mutant enzyme F206Y is 2.4fold higher than activity with wild-type enzyme
742292
Escherichia coli
D-isoserine + succinate + CO2
-
-
-
?
butyric acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxy-4-butyric acid + succinate + CO2
-
-
-
?
propionic acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxypropionic acid + succinate + CO2
-
-
-
?
valeric acid + 2-oxoglutarate + O2
mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme
742292
Escherichia coli
2-hydroxyvaleric acid + succinate + CO2
-
-
-
?
Other publictions for EC 1.14.11.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743152
Alvarez-Barcia
Atom tunneling in the hydroxy ...
Escherichia coli
J. Phys. Chem. B
121
5347-5354
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742292
Wetzl
-
alpha-Hydroxylation of carbox ...
Escherichia coli
ChemCatChem
8
1361-1366
2016
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742651
Henderson
Calorimetric assessment of Fe ...
Escherichia coli
Inorg. Chem.
54
2278-2283
2015
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725787
Casey
Measuring the orientation of t ...
Escherichia coli
J. Phys. Chem. B
117
10384-10394
2013
-
-
-
1
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
723940
Matsuda
A simple assay of taurine conc ...
Escherichia coli
Anal. Biochem.
427
121-123
2012
-
1
1
-
-
-
1
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
724974
Knauer
The Fe(II)/alpha-ketoglutarate ...
Escherichia coli, Pseudomonas putida, Pseudomonas putida KT 2240
FEBS J.
279
816-831
2012
-
-
1
1
-
-
-
-
-
2
6
3
-
3
-
-
-
-
-
-
2
-
3
3
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
2
6
3
-
-
-
-
-
-
2
-
3
3
-
-
-
1
-
-
-
-
-
2
2
-
-
-
712249
Ye
Cryoreduction of the NO-adduct ...
Escherichia coli
J. Am. Chem. Soc.
132
4739-4751
2010
-
-
-
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697475
de Visser
-
Elucidating enzyme mechanism a ...
Homo sapiens
Coord. Chem. Rev.
253
754-768
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697518
Ye
Quantum chemical studies of C- ...
Escherichia coli
Curr. Opin. Chem. Biol.
13
89-98
2009
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
701001
McCusker
Modular behavior of tauD provi ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
106
19791-19795
2009
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
701264
McCusker
-
Facile synthesis of 1,1-[2H2]- ...
Escherichia coli
Tetrahedron Lett.
50
611-613
2009
-
1
1
-
-
-
-
7
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
4
4
688611
Godfrey
Comparative quantum mechanics/ ...
Escherichia coli
J. Phys. Chem. A
112
2464-2468
2008
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698476
Mirica
18O kinetic isotope effects in ...
Bacteria
J. Am. Chem. Soc.
130
8122-8123
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
673044
de Visser
Can the peroxosuccinate comple ...
Escherichia coli
Chem. Commun. (Camb. )
2007
171-173
2007
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685159
Muthukumaran
Probing the iron-substrate ori ...
Escherichia coli
Biochemistry
46
5951-5959
2007
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687079
Grzyska
Cr(II) reactivity of taurine/a ...
Escherichia coli
Inorg. Chem.
46
10087-10092
2007
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687279
Neidig
CD and MCD of CytC3 and taurin ...
Escherichia coli
J. Am. Chem. Soc.
129
14224-14231
2007
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687288
Sinnecker
Spectroscopic and computationa ...
Escherichia coli
J. Am. Chem. Soc.
129
6168-6179
2007
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688192
Grzyska
Metal ligand substitution and ...
Escherichia coli
J. Inorg. Biochem.
101
797-808
2007
-
-
-
-
18
-
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
671301
Luo
An assay for Fe(II)/2-oxogluta ...
Escherichia coli
Anal. Biochem.
353
69-74
2006
-
-
-
-
2
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
674930
Koehntop
Self-hydroxylation of taurine/ ...
Escherichia coli
J. Biol. Inorg. Chem.
11
63-72
2006
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658130
Price
Kinetic dissection of the cata ...
Escherichia coli
Biochemistry
44
8138-8147
2005
-
-
-
-
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671717
Kalliri
Kinetic and spectroscopic inve ...
Escherichia coli
Biochem. Biophys. Res. Commun.
338
191-197
2005
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
4
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673443
Bollinger
-
Mechanism of taurine: alpha-ke ...
Escherichia coli
Eur. J. Inorg. Chem.
2005
4245-4254
2005
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658975
Proshlyakov
Direct detection of oxygen int ...
Escherichia coli
J. Am. Chem. Soc.
126
1022-1023
2004
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658984
Riggs-Gelasco
EXAFS spectroscopic evidence f ...
Escherichia coli
J. Am. Chem. Soc.
126
8108-8109
2004
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657996
O'Brien
Substrate-induced conformation ...
Escherichia coli
Biochemistry
42
5547-5554
2003
-
-
1
1
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657933
Elkins
X-ray crystal structure of Esc ...
Escherichia coli
Biochemistry
41
5185-5192
2002
-
-
-
1
-
-
-
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680611
Kahnert
Characterization of a sulfur-r ...
Pseudomonas putida, Pseudomonas putida S-313
J. Biol. Chem.
275
31661-31667
2000
2
-
1
-
-
-
4
-
-
2
3
-
-
5
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
-
-
2
3
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285263
Eichhorn
Characterization of alpha-keto ...
Escherichia coli
J. Biol. Chem.
272
23031-23036
1997
1
-
1
-
-
-
4
7
-
2
3
-
-
3
-
-
1
-
-
-
2
2
7
1
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
7
-
2
3
-
-
-
-
1
-
-
2
2
7
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-