Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F206Y | the conversions of the improved catalyst increases by at least 140% compared to that of the wild-type enzyme and shows broadened substrate scope | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P37610 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminobutyric acid + 2-oxoglutarate + O2 | activity with mutant enzyme F206Y is 4.7fold higher than activity with wild-type enzyme | Escherichia coli | D-2-hydroxy-4-aminobutyric acid + succinate + CO2 | - |
? | |
5-aminovaleric acid + 2-oxoglutarate + O2 | activity with mutant enzyme F206Y is 4.4fold higher than activity with wild-type enzyme | Escherichia coli | D-2-hydroxy-5-aminovaleric acid + succinate + CO2 | - |
? | |
6-aminocaproic acid + 2-oxoglutarate + O2 | mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme | Escherichia coli | D-2-hydroxy-6-aminocaproic acid + succinate + CO2 | - |
? | |
alpha-methyl-beta-alanine + 2-oxoglutarate + O2 | mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme | Escherichia coli | 3-amino-2-hydroxy-2-methylpropanoic acid + succinate + CO2 | - |
? | |
beta-alanine + 2-oxoglutarate + O2 | activity with mutant enzyme F206Y is 2.4fold higher than activity with wild-type enzyme | Escherichia coli | D-isoserine + succinate + CO2 | - |
? | |
butyric acid + 2-oxoglutarate + O2 | mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme | Escherichia coli | 2-hydroxybutyric acid + succinate + CO2 | - |
? | |
propionic acid + 2-oxoglutarate + O2 | mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme | Escherichia coli | 2-hydroxypropionic acid + succinate + CO2 | - |
? | |
valeric acid + 2-oxoglutarate + O2 | mutant enzyme F206Y shows low activity. No activity with the wild-type enzyme | Escherichia coli | 2-hydroxyvaleric acid + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TauD | - |
Escherichia coli |