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Literature summary for 1.13.12.24 extracted from

  • Malikova, N.P.; Eremeeva, E.V.; Gulnov, D.V.; Natashin, P.V.; Nemtseva, E.V.; Vysotski, E.S.
    Specific activities of hydromedusan Ca2+-regulated photoproteins (2022), Photochem. Photobiol., 98, 275-283 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Aequorea victoria
expression in Escherichia coli BL21 Obelia longissima
expression in Escherichia coli BL21 Obelia geniculata
expression in Escherichia coli BL21 Clytia gregaria
expression in Escherichia coli BL21 Mitrocoma cellularia

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-regulated photoprotein Aequorea victoria
Ca2+ Ca2+-regulated photoprotein Obelia longissima
Ca2+ Ca2+-regulated photoprotein Obelia geniculata
Ca2+ Ca2+-regulated photoprotein Clytia gregaria
Ca2+ Ca2+-regulated photoprotein Mitrocoma cellularia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Aequorea victoria
-
[excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Obelia longissima
-
[excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Obelia geniculata
-
[excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Clytia gregaria
-
[excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Mitrocoma cellularia
-
[excited state blue fluorescent protein] + CO2
-
?

Organism

Organism UniProt Comment Textmining
Aequorea victoria P07164
-
-
Clytia gregaria Q08121
-
-
Mitrocoma cellularia P39047
-
-
Obelia geniculata Q8T6Z0
-
-
Obelia longissima Q27709
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aequorea victoria
-
Obelia longissima
-
Obelia geniculata
-
Clytia gregaria
-
Mitrocoma cellularia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
Aequorea victoria [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
Obelia longissima [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
Obelia geniculata [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
Clytia gregaria [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
Mitrocoma cellularia [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Aequorea victoria [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Obelia longissima [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Obelia geniculata [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Clytia gregaria [excited state blue fluorescent protein] + CO2
-
?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the specific bioluminescence activities of five recombinant hydromedusan Ca2+-regulated photoproteins - aequorin, mitrocomin, clytin and obelins from Obelia longissima and Obelia geniculata is compared. Along with bioluminescence spectra, kinetics of light emission reactions and sensitivities to calcium, these photoproteins also differ in specific activities and consequently in quantum yields of biox02luminescent reactions. The highest specific activities are found for obelins and mitrocomin, whereas those of aequorin and clytin are lower Mitrocoma cellularia [excited state blue fluorescent protein] + CO2
-
?

Synonyms

Synonyms Comment Organism
aequorin
-
Aequorea victoria
clytin
-
Clytia gregaria
mitrocomin
-
Mitrocoma cellularia
obelin
-
Obelia longissima
obelin
-
Obelia geniculata