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Literature summary for 1.13.12.24 extracted from

  • Molakarimi, M.; Gorman, M.; Mohseni, A.; Pashandi, Z.; Taghdir, M.; Naderi-Manesh, H.; Sajedi, R.; Parker, M.
    Reaction mechanism of the bioluminescent protein mnemiopsin1 revealed by X-ray crystallography and QM/MM simulations (2019), J. Biol. Chem., 294, 20-27 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)Star cells Mnemiopsis leidyi

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, crystal structure of Cd2+-loaded apo-mnemiopsin1 at 2.15 A resolution. The structure reveals that mnemiopsin1 has a two-domain fold with four alpha-helices in each domain Mnemiopsis leidyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 gel filtartion Mnemiopsis leidyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ Mnemiopsis leidyi
-
 [excited state blue fluorescent protein] + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Mnemiopsis leidyi E0WVU4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mnemiopsis leidyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[apoaequorin] + coelenterazine + O2 + 3 Ca2+
-
 Mnemiopsis leidyi [excited state blue fluorescent protein] + CO2
-
 ?
[apoaequorin] + coelenterazine + O2 + 3 Ca2+ the reaction mechanism of the bioluminescent protein mnemiopsin1 is revealed by X-ray crystallography and QM/MM simulations. A water molecule in the coelenteramide binding cavity is identified that forms a hydrogen bond with the amide nitrogen atom of coelenteramide, which, in turn, is hydrogen-bonded via another water molecule to Tyr-131. This observation supports the hypothesis that the function of the coelenteramide-bound water molecule is to catalyze the 2-hydroperoxycoelenterazine decarboxylation reaction by protonation of a dioxetanone anion, thereby triggering the bioluminescence reaction Mnemiopsis leidyi [excited state blue fluorescent protein] + CO2
-
 ?

Synonyms

Synonyms Comment Organism
mnemiopsin1
-
 Mnemiopsis leidyi