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Literature summary for 1.13.12.16 extracted from
- Evidence for a transient peroxynitro acid in the reaction catalyzed by nitronate monooxygenase with propionate 3-nitronate (2013), Biochemistry, 52, 2694-2704.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
propionate 3-nitronate | pH 5.5, 30°C | Cyberlindnera saturnus |  |
| 0.08 | - |
O2 | pH 5.5, 30°C | Cyberlindnera saturnus | |
| 4 | - |
ethyl nitronate | pH 8, 30°C | Cyberlindnera saturnus | |
| 4 | - |
ethyl nitronate | pH 6, 30°C | Cyberlindnera saturnus | |
| 10 | - |
ethyl nitronate | pH 11, 30°C | Cyberlindnera saturnus | |
| 20 | - |
ethyl nitronate | pH 5.5, 30°C | Cyberlindnera saturnus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera saturnus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl nitronate + O2 | - |
Cyberlindnera saturnus | ? + nitrite | - |
? | |
| propionate 3-nitronate + O2 | - |
Cyberlindnera saturnus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitronate monooxygenase | - |
Cyberlindnera saturnus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Cyberlindnera saturnus |
| 30 | - |
assay at | Cyberlindnera saturnus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40 | - |
ethyl nitronate | pH 11, 30°C | Cyberlindnera saturnus | |
| 40 | - |
ethyl nitronate | pH 6, 30°C | Cyberlindnera saturnus | |
| 840 | - |
ethyl nitronate | pH 8, 30°C | Cyberlindnera saturnus | |
| 860 | - |
propionate 3-nitronate | pH 5.5, 30°C | Cyberlindnera saturnus | |
| 4100 | - |
ethyl nitronate | pH 5.5, 30°C | Cyberlindnera saturnus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Cyberlindnera saturnus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41 | - |
ethyl nitronate | pH 11, 30°C | Cyberlindnera saturnus | |
| 46 | - |
ethyl nitronate | pH 6, 30°C | Cyberlindnera saturnus | |
| 200 | - |
ethyl nitronate | pH 8, 30°C | Cyberlindnera saturnus | |
| 300 | - |
ethyl nitronate | pH 5.5, 30°C | Cyberlindnera saturnus | |
| 11000 | - |
O2 | pH 5.5, 30°C | Cyberlindnera saturnus | |
| 14330 | - |
propionate 3-nitronate | pH 5.5, 30°C | Cyberlindnera saturnus | |
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