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Literature summary for 1.13.12.16 extracted from

  • Kido, T.; Soda, K.
    Oxidation of anionic nitroalkanes by flavoenzymes, and participation of superoxide anion in the catalysis (1984), Arch. Biochem. Biophys., 234, 468-475.
    View publication on PubMed
Search for more literature for 1.13.12.16

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy-2,2,6,6-tetramethyl-piperidinooxy radical
-
 Cyberlindnera mrakii
cytochrome c O2 scavenger Cyberlindnera mrakii
dithiothreitol
-
 Cyberlindnera mrakii
epinephrine
-
 Cyberlindnera mrakii
GSH
-
 Cyberlindnera mrakii
additional information not affected by hydroxyl radical scavengers such as mannitol Cyberlindnera mrakii
NaBH4
-
 Cyberlindnera mrakii
NADH O2 scavenger Cyberlindnera mrakii
Nitro blue tetrazolium
-
 Cyberlindnera mrakii
Superoxide dismutase Cu and Zn-superoxide dismutase of bovine blood, Mn-superoxide dismutases of bacilli, Fe-superoxide dismutase of Serratia marcescens Cyberlindnera mrakii
Tiron i.e. pyrocatechol-3,5-disulfonate disodium salt Cyberlindnera mrakii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.61
-
 2-Nitropropane anionic form of substrate Cyberlindnera mrakii
1.61
-
 propyl-2-nitronate anionic Cyberlindnera mrakii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of Cu2+ and Zn2+ shows no effect Cyberlindnera mrakii

Organism

Organism UniProt Comment Textmining
Cyberlindnera mrakii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cyberlindnera mrakii

Reaction

Reaction Comment Organism Reaction ID
ethylnitronate + O2 = acetaldehyde + nitrite + other products superoxide as reactive intermediate Cyberlindnera mrakii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxybutyl-2-nitronate + O2 anionic, expression on the basis of the reactivity of propyl-2-nitronate with 2-nitropropane dioxygenase: 31.5 Cyberlindnera mrakii ? + HNO2
-
 ?
2-hydroxybutyl-3-nitronate + O2 anionic, expression on the basis of the reactivity of propyl-2-nitronate with 2-nitropropane dioxygenase: 26.7 Cyberlindnera mrakii ? + HNO2
-
 ?
2-hydroxypentyl-3-nitronate + O2 anionic, expression on the basis of the reactivity of propyl-2-nitronate with 2-nitropropane dioxygenase: 32.3 Cyberlindnera mrakii ? + HNO2
-
 ?
2-nitropropane + O2 superoxide as reactive intermediate Cyberlindnera mrakii acetone + HNO2
-
 ?
ethylnitronate + O2 anionic, expression on the basis of the reactivity of propyl-2-nitronate with 2-nitropropane dioxygenase: 32.5 Cyberlindnera mrakii acetaldehyde + HNO2
-
 ?
nitroethane + O2
-
 Cyberlindnera mrakii acetaldehyde + HNO2
-
 ?
propyl-2-nitronate + O2 anionic Cyberlindnera mrakii acetone + HNO2
-
 ?
propylnitronate + O2 anionic, expression on the basis of the reactivity of propyl-2-nitronate with 2-nitropropane dioxygenase: 57.9 Cyberlindnera mrakii ? + HNO2
-
 ?

Synonyms

Synonyms Comment Organism
2-nitropropane dioxygenase
-
 Cyberlindnera mrakii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Cyberlindnera mrakii

Cofactor

Cofactor Comment Organism Structure
FAD dependent Cyberlindnera mrakii