Crystallization (Comment) | Organism |
---|---|
structure of variant G95C | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
G95C | variant is able to form a cysteine-tyrosine crosslink homologous to that found in mammalian cysteine dioxygenases. Activity of this variant is severely impaired | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.0, 37°C | Pseudomonas aeruginosa | |
0.6 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.7 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.0, 37°C | Pseudomonas aeruginosa | |
0.8 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.9 | - |
3-Mercaptopropanoate | mutant G95C, PIPES buffer pH 6.5, 37°C | Pseudomonas aeruginosa | |
0.9 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.1, 37°C | Pseudomonas aeruginosa | |
1 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.5, 37°C | Pseudomonas aeruginosa | |
1.4 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.5, 37°C | Pseudomonas aeruginosa | |
4 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 4.7, 37°C | Pseudomonas aeruginosa | |
5 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 5.5, 37°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I0N5 | - |
- |
Pseudomonas aeruginosa DSM 22644 | Q9I0N5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopropanoate + O2 | - |
Pseudomonas aeruginosa | 3-sulfinopropanoate | - |
? | |
3-mercaptopropanoate + O2 | - |
Pseudomonas aeruginosa DSM 22644 | 3-sulfinopropanoate | - |
? | |
L-cysteine + O2 | reaction of EC 1.13.11.20 | Pseudomonas aeruginosa | 3-sulfinoalanine | - |
? | |
L-cysteine + O2 | reaction of EC 1.13.11.20 | Pseudomonas aeruginosa DSM 22644 | 3-sulfinoalanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NP_251292 | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
3-Mercaptopropanoate | mutant G95C, PIPES buffer pH 6.5, 37°C | Pseudomonas aeruginosa | |
0.03 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.5, 37°C | Pseudomonas aeruginosa | |
0.05 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.0, 37°C | Pseudomonas aeruginosa | |
0.1 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.1 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.11 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 4.7, 37°C | Pseudomonas aeruginosa | |
0.13 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.0, 37°C | Pseudomonas aeruginosa | |
0.19 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.5, 37°C | Pseudomonas aeruginosa | |
0.22 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 5.5, 37°C | Pseudomonas aeruginosa | |
0.23 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.1, 37°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the monoprotonated ES complexes with 3-mercaptopropionic acid and cysteine have different pKs. At higher pH, kcat decreases sigmoidally with a similar pK regardless of substrate. Loss of reactivity at high pH is attributed to deprotonation of tyrosine 159 and its influence on dioxygen binding | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | the monoprotonated ES complexes with 3-mercaptopropionic acid and cysteine have different pKs. At higher pH, kcat decreases sigmoidally with a similar pK regardless of substrate. Loss of reactivity at high pH is attributed to deprotonation of tyrosine 159 and its influence on dioxygen binding. A mechanism model shows deprotonation of tyrosine 159 both blocks oxygen binding and concomitantly promotes cystine formation | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 4.7, 37°C | Pseudomonas aeruginosa | |
0.05 | - |
3-Mercaptopropanoate | wild-type, acetate buffer pH 5.5, 37°C | Pseudomonas aeruginosa | |
0.12 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.18 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 7.5, 37°C | Pseudomonas aeruginosa | |
0.18 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.5, 37°C | Pseudomonas aeruginosa | |
0.2 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.5, 37°C | Pseudomonas aeruginosa | |
0.26 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 6.1, 37°C | Pseudomonas aeruginosa | |
0.27 | - |
3-Mercaptopropanoate | wild-type, PIPES buffer pH 7.0, 37°C | Pseudomonas aeruginosa | |
0.7 | - |
3-Mercaptopropanoate | wild-type, TRIS buffer pH 8.0, 37°C | Pseudomonas aeruginosa |