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Literature summary for 1.13.11.91 extracted from

  • Wenning, L.; Stoeveken, N.; Wuebbeler, J.H.; Steinbuechel, A.
    Substrate and cofactor range differences of two cysteine dioxygenases from Ralstonia eutropha H16 (2015), Appl. Environ. Microbiol., 82, 910-921 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.91

Activating Compound

Activating Compound Comment Organism Structure
ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.05 mM, 80% of initial activity, 0.1 mM, 61% of initial activity Cupriavidus necator
ethylxanthate 0.002 mM, 110% of initial activity, 0.004 mM, 89% of initial activity Cupriavidus necator
additional information addition of cysteine results in a minor decrease of activity Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.7
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron content of 0.38 mol Fe(II) per mol of enzyme Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46800
-
 gel filtration Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q0K029
-
-
Cupriavidus necator DSM 428 Q0K029
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
 pH 6.5, 30°C Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-mercaptopropanoate + O2
-
 Cupriavidus necator 3-sulfinopropanoate
-
 ?
L-cysteine + O2 reaction of EC 1.13.11.20 Cupriavidus necator 3-sulfinoalanine
-
 ?
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator ?
-
-
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator DSM 428 ?
-
-
additional information 3-mercaptopropanoate is preferred over cysteine. No substrate: cysteamine Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
-

Subunits

Subunits Comment Organism
dimer 2 * 22580, calculated from sequence Cupriavidus necator

Synonyms

Synonyms Comment Organism
CdoA
-
 Cupriavidus necator
H16_B1863
-
 Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.82
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Cupriavidus necator

pI Value

Organism Comment pI Value Maximum pI Value
Cupriavidus necator calculated from sequence
-
 5.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.144
-
 3-Mercaptopropanoate pH 6.5, 30°C Cupriavidus necator