Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method at 20°C, crystal structure for the resting form of the enzyme (NicX), as well as a complex structure wherein 2,5-hydroxy-pyridine and N-formylmaleamic acid are trapped in different subunits. The resting state structure displays an octahedral coordination for Fe(II) with two histidine residues (His265 and His318), a serine residue (Ser302), a carboxylate ligand (Asp320), and two water molecules. 2,5-Hydroxy-pyridine does not bind as a ligand to Fe(II), yet its interactions with Leu104 and His105 function to guide and stabilize the substrate to the appropriate position to initiate the reaction | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme uses a mononuclear non-heme iron enzyme to catalyze the oxidative pyridine ring cleavage reaction | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxypyridine + O2 | Pseudomonas putida | - |
N-formylmaleamic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q88FY1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxypyridine + O2 | - |
Pseudomonas putida | N-formylmaleamic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,5-dihydroxypyridine dioxygenase | - |
Pseudomonas putida |
NicX | - |
Pseudomonas putida |