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Literature summary for 1.13.11.87 extracted from

  • Birke, J.; Röther, W.; Jendrossek, D.
    RoxB is a novel type of rubber oxygenase that combines properties of rubber oxygenase RoxA and latex clearing protein (Lcp) (2017), Appl. Environ. Microbiol., 83, e00721 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.87

Application

Application Comment Organism
degradation enzyme exerts a synergistic effect on the efficiency of polyisoprene cleavage by rubber oxidase RoxA, EC 1.13.11.85 Xanthomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Diethyl dithiocarbamate
-
 Xanthomonas sp.
imidazole
-
 Xanthomonas sp.
additional information not inhibitory: EDTA up to 10 mM, carbon monoxide Xanthomonas sp.
Pyridine
-
 Xanthomonas sp.
SDS partial inhibition Xanthomonas sp.

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. A0A1S6Q8F9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification calculated molecular mass is 73800 Da for precursor protein, and 70300 Da for mature protein Xanthomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 pH 7.0, 23°C Xanthomonas sp.
5.7
-
 pH 7.0, 30°C Xanthomonas sp.
6.4
-
 pH 7.0, 37°C Xanthomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2
-
 Xanthomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 73800, calculated for precursor protein, x * 70300, calculated for mature protein, x * 70300, SDS-PAGE Xanthomonas sp.

Synonyms

Synonyms Comment Organism
RoxB
-
 Xanthomonas sp.
rubber oxygenase B
-
 Xanthomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Xanthomonas sp.

Cofactor

Cofactor Comment Organism Structure
heme presence of 2 c-type hemes, presence of two c-type heme binding motifs (C192HAC195H and C391ASC394H) Xanthomonas sp.

Expression

Organism Comment Expression
Xanthomonas sp. expression is upregulated during growth on polyisoprene up

General Information

General Information Comment Organism
physiological function synergistic effect of the simultaneous presence of RoxAXsp and RoxBXsp on the efficiency of polyisoprene cleavage Xanthomonas sp.