BRENDA - Enzyme Database show
show all sequences of 1.13.11.87

Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms

Birke, J.; Jendrossek, D.; Appl. Environ. Microbiol. 80, 5012-5020 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of Lcp of Streptomyces sp. K30 in a DELTAroxA background of Xanthomonas sp. strain 35Y
Streptomyces sp. K30
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-tocopherol
1 mM, 40% inhibition
Streptomyces sp. K30
ascorbate
1 mM, 20% inhibition
Streptomyces sp. K30
carotene
1 mM, 40% inhibition
Streptomyces sp. K30
diethyldithiocarbamate
1 mM, 50% inhibition
Streptomyces sp. K30
dithiothreitol
10 mM, 90% inhibition
Streptomyces sp. K30
EDTA
1 mM, 30% inhibition
Streptomyces sp. K30
ethanol
5%, 10% inhibition
Streptomyces sp. K30
imidazole
inhibits by 35% and 85% at 1 mM and 10 mM
Streptomyces sp. K30
Phenanthroline
1 mM, 10% inhibition
Streptomyces sp. K30
SDS
0.1%, complete inhibition
Streptomyces sp. K30
squalene
1 mM, 10% inhibition
Streptomyces sp. K30
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Streptomyces sp. K30
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no indication for the presence of iron, other metals, or cofactors in Lcp
Streptomyces sp. K30
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Streptomyces sp. K30
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp. K30
Q3L8N0
-
-
Purification (Commentary)
Commentary
Organism
-
Streptomyces sp. K30
Storage Stability
Storage Stability
Organism
almost stable during storage on ice
Streptomyces sp. K30
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cis-1,4-polyisoprene + O2
Lcp degrades polyisoprene via endo-type cleavage to tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
744096
Streptomyces sp. K30
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Streptomyces sp. K30
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
23
-
Lcp activity decreases by incubation at 23°C by 70% within the 22 h of incubation time
Streptomyces sp. K30
37
-
Lcp activity decreases by incubation at 37°C by 80% within the 22 h of incubation time
Streptomyces sp. K30
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no indication for the presence of iron, other metals, or cofactors in Lcp
Streptomyces sp. K30
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of Lcp of Streptomyces sp. K30 in a DELTAroxA background of Xanthomonas sp. strain 35Y
Streptomyces sp. K30
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no indication for the presence of iron, other metals, or cofactors in Lcp
Streptomyces sp. K30
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-tocopherol
1 mM, 40% inhibition
Streptomyces sp. K30
ascorbate
1 mM, 20% inhibition
Streptomyces sp. K30
carotene
1 mM, 40% inhibition
Streptomyces sp. K30
diethyldithiocarbamate
1 mM, 50% inhibition
Streptomyces sp. K30
dithiothreitol
10 mM, 90% inhibition
Streptomyces sp. K30
EDTA
1 mM, 30% inhibition
Streptomyces sp. K30
ethanol
5%, 10% inhibition
Streptomyces sp. K30
imidazole
inhibits by 35% and 85% at 1 mM and 10 mM
Streptomyces sp. K30
Phenanthroline
1 mM, 10% inhibition
Streptomyces sp. K30
SDS
0.1%, complete inhibition
Streptomyces sp. K30
squalene
1 mM, 10% inhibition
Streptomyces sp. K30
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Streptomyces sp. K30
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no indication for the presence of iron, other metals, or cofactors in Lcp
Streptomyces sp. K30
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Streptomyces sp. K30
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces sp. K30
Storage Stability (protein specific)
Storage Stability
Organism
almost stable during storage on ice
Streptomyces sp. K30
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cis-1,4-polyisoprene + O2
Lcp degrades polyisoprene via endo-type cleavage to tetra-C20 and higher oligo-isoprenoids with aldehyde and keto end groups
744096
Streptomyces sp. K30
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, SDS-PAGE
Streptomyces sp. K30
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
23
-
Lcp activity decreases by incubation at 23°C by 70% within the 22 h of incubation time
Streptomyces sp. K30
37
-
Lcp activity decreases by incubation at 37°C by 80% within the 22 h of incubation time
Streptomyces sp. K30
Other publictions for EC 1.13.11.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746868
Jendrossek
Rubber oxygenases ...
Rhizobacter gummiphilus, Rhizobacter gummiphilus NS21
Appl. Microbiol. Biotechnol.
103
125-142
2019
-
-
-
-
-
-
-
-
-
-
2
-
-
5
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746860
Birke
Rhizobacter gummiphilus NS21 ...
Rhizobacter gummiphilus, Rhizobacter gummiphilus NS21
Appl. Microbiol. Biotechnol.
102
10245-10257
2018
-
-
1
-
-
-
-
-
-
-
2
2
-
7
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746839
Birke
RoxB Is a Novel Type of Rubbe ...
Xanthomonas sp. 35Y
Appl. Environ. Microbiol.
83
e00721-17
2017
-
-
1
-
-
-
6
-
-
-
1
-
-
3
-
1
1
-
-
-
3
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
-
1
-
-
-
1
1
-
-
3
-
1
-
-
-
-
-
1
1
-
-
1
1
1
1
-
-
748256
Linh
Characterization and function ...
Nocardia farcinica, Nocardia farcinica DSM 43665T
J. Biosci. Bioeng.
123
412-418
2017
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
748572
Roether
Production of functionalized ...
Streptomyces sp. K30, Xanthomonas sp. 35Y
Microb. Biotechnol.
10
1426-1433
2017
-
2
2
-
-
-
-
-
-
-
-
2
-
4
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749354
Ilcu
Structural and functional ana ...
Streptomyces sp. K30
Sci. Rep.
7
6179
2017
-
-
1
1
4
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
746838
Roether
Cleavage of rubber by the lat ...
Streptomyces sp. K30
Appl. Environ. Microbiol.
82
6593-6602
2016
-
-
-
-
9
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747416
Watcharakul
Biochemical and spectroscopic ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous RPK1
BMC Microbiol.
16
92
2016
-
-
1
-
-
-
2
-
-
-
2
-
-
6
-
1
1
-
-
2
2
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
2
-
-
-
1
1
-
2
2
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
744101
Birke
Latex clearing protein (Lcp) ...
Streptomyces sp. K30
Appl. Environ. Microbiol.
81
3793-3799
2015
-
-
1
-
-
-
6
-
-
1
1
-
-
4
-
-
1
-
-
1
2
-
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
-
-
1
1
-
-
-
-
1
-
1
2
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
744096
Birke
Rubber oxygenase and latex cl ...
Streptomyces sp. K30
Appl. Environ. Microbiol.
80
5012-5020
2014
-
-
1
-
-
-
11
-
1
1
1
-
-
5
-
-
1
-
-
-
-
1
1
1
-
-
2
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
11
-
-
1
1
1
-
-
-
-
1
-
-
-
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
746820
Hiessl
Involvement of two latex-clea ...
Gordonia polyisoprenivorans, Gordonia polyisoprenivorans VH2
Appl. Environ. Microbiol.
78
2874-2887
2012
-
-
-
-
-
-
-
-
-
-
-
2
-
2
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4
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2
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-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748618
Yikmis
Importance of the latex-clear ...
Streptomyces sp. K30
MicrobiologyOpen
1
13-24
2012
-
-
1
-
-
-
-
-
1
-
-
1
-
8
-
-
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-
-
-
-
-
2
-
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1
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1
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1
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-
2
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-
-
-
-
-
-
-
-
2
2
-
-
-
746818
Yikmis
Secretion and transcriptional ...
Streptomyces sp. K30
Appl. Environ. Microbiol.
74
5373-5382
2008
-
-
-
-
-
-
-
-
-
-
-
-
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6
-
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1
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1
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-
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-
-
1
1
1
1
-
-
746816
Ibrahim
Identification of poly(cis-1, ...
Nocardia farcinica, Nocardia farcinica E1
Appl. Environ. Microbiol.
72
3375-3382
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
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-
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4
-
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1
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2
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4
-
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-
-
-
-
-
-
1
1
-
-
-
744090
Braaz
Heme-dependent rubber oxygena ...
Xanthomonas sp. 35Y
Appl. Environ. Microbiol.
71
2473-2478
2005
-
-
-
-
-
-
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2
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1
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2
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1
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1
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1
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1
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2
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1
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1
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-
747794
Jendrossek
Sequence analysis of a gene p ...
Xanthomonas sp.
FEMS Microbiol. Lett.
224
61-65
2003
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1
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1
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1
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