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Literature summary for 1.13.11.80 extracted from

  • Li, K.; Fielding, E.N.; Condurso, H.L.; Bruner, S.D.
    Probing the structural basis of oxygen binding in a cofactor-independent dioxygenase (2017), Acta Crystallogr. Sect. D, 73, 573-580.
    View publication on PubMed
Search for more literature for 1.13.11.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces toyocaensis

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzymes A319C and V425T in complex with substrate, hanging drop vapor diffusion method, using 140 mM ammonium acetate, 14% (w/v) PEG 4000, 100 mM sodium citrate pH 5.6 Streptomyces toyocaensis

Protein Variants

Protein Variants Comment Organism
A319C the catalytic efficiency of the mutant is about 1.5% of that of the wild type protein Streptomyces toyocaensis
V425T the mutant shows reduced activity compared to the wild type enzyme Streptomyces toyocaensis

Inhibitors

Inhibitors Comment Organism Structure
dihydroxyphenylacetyl-amine-CoA
-
 Streptomyces toyocaensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 (3,5-dihydroxyphenyl)acetyl-CoA mutant enzyme A319C, at pH 7.5 and 24°C Streptomyces toyocaensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3,5-dihydroxyphenyl)acetyl-CoA + O2 Streptomyces toyocaensis
-
 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?
(3,5-dihydroxyphenyl)acetyl-CoA + O2 Streptomyces toyocaensis A47934
-
 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces toyocaensis Q8KLK7
-
-
Streptomyces toyocaensis A47934 Q8KLK7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Streptomyces toyocaensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3,5-dihydroxyphenyl)acetyl-CoA + O2
-
 Streptomyces toyocaensis 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?
(3,5-dihydroxyphenyl)acetyl-CoA + O2
-
 Streptomyces toyocaensis A47934 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Streptomyces toyocaensis

Synonyms

Synonyms Comment Organism
DpgC
-
 Streptomyces toyocaensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.095
-
 (3,5-dihydroxyphenyl)acetyl-CoA mutant enzyme A319C, at pH 7.5 and 24°C Streptomyces toyocaensis

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is cofactor-independent Streptomyces toyocaensis