BRENDA - Enzyme Database
show all sequences of 1.13.11.77

Production of 10S-hydroxy-8(E)-octadecenoic acid from oleic acid by whole recombinant Escherichia coli cells expressing 10S-dioxygenase from Nostoc punctiforme PCC 73102 with the aid of a chaperone

Kim, M.J.; Seo, M.J.; Shin, K.C.; Oh, D.K.; Biotechnol. Lett. 39, 133-139 (2017)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
chaperone Gro7
activates the enzyme about 200%
Nostoc punctiforme
Cloned(Commentary)
Cloned (Commentary)
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with chaperone Gro7
Nostoc punctiforme
Engineering
Protein Variants
Commentary
Organism
additional information
optimization of conditions for 10S-hydroxy-8(E)-octadecenoic acid production from oleic acid by recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone plasmid. Optimal conditions are pH 9.0, 35°C, 15 % v/v dimethyl sulfoxide, 40 g cells/l, and 10 g oleic acid/l. The activity of recombinant cells expressing 10S-dioxygenase is increased by 200% with the aid of chaperone Gro7. Production of 10S-hydroxy-8(E)-octadecenoic acid, 10S-hydroxy-8,12(E,Z)-octadecadienoic acid, and 10S-hydroxy-8,12,15(E,Z,Z)-octadecatrienoic acid from unsaturated fatty acids including oleic acid, linoleic acid, and a-linolenic acid, respectively. The soluble 10S-dioxygenase content in the crude extract of recombinant cells co-expressing the 10S-dioxygenase gene with pGro7 is higher than that of recombinant cells co-expressing pKJE7 or pG-KJE8. Production of 360 mg/g/h 10S-hydroxy-8(E)-octadecenoic acid from oleic acid
Nostoc punctiforme
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.096
-
alpha-linolenate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
0.236
-
linoleate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
0.345
-
oleate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alpha-linolenate + O2
Nostoc punctiforme
-
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
linoleate + O2
Nostoc punctiforme
-
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
oleate + O2
Nostoc punctiforme
preferred substrate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nostoc punctiforme
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
Nostoc punctiforme
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.096
-
purified recombinant enzyme, substrate alpha-linolenate, pH 9.0, 35°C
Nostoc punctiforme
0.236
-
purified recombinant enzyme, substrate linoleate, pH 9.0, 35°C
Nostoc punctiforme
0.345
-
purified recombinant enzyme, substrate oleate, pH 9.0, 35°C
Nostoc punctiforme
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
alpha-linolenate + O2
-
744582
Nostoc punctiforme
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
linoleate + O2
-
744582
Nostoc punctiforme
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
additional information
optimization of assay conditions, overview
744582
Nostoc punctiforme
?
-
-
-
-
oleate + O2
preferred substrate
744582
Nostoc punctiforme
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 63000, recombinant His-tagged enzyme, SDS-PAGE
Nostoc punctiforme
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
recombinant His-tagged enzyme
Nostoc punctiforme
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
recombinant His-tagged enzyme
Nostoc punctiforme
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
chaperone Gro7
activates the enzyme about 200%
Nostoc punctiforme
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with chaperone Gro7
Nostoc punctiforme
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
optimization of conditions for 10S-hydroxy-8(E)-octadecenoic acid production from oleic acid by recombinant Escherichia coli cells expressing Nostoc punctiforme 10S-dioxygenase with the aid of a chaperone plasmid. Optimal conditions are pH 9.0, 35°C, 15 % v/v dimethyl sulfoxide, 40 g cells/l, and 10 g oleic acid/l. The activity of recombinant cells expressing 10S-dioxygenase is increased by 200% with the aid of chaperone Gro7. Production of 10S-hydroxy-8(E)-octadecenoic acid, 10S-hydroxy-8,12(E,Z)-octadecadienoic acid, and 10S-hydroxy-8,12,15(E,Z,Z)-octadecatrienoic acid from unsaturated fatty acids including oleic acid, linoleic acid, and a-linolenic acid, respectively. The soluble 10S-dioxygenase content in the crude extract of recombinant cells co-expressing the 10S-dioxygenase gene with pGro7 is higher than that of recombinant cells co-expressing pKJE7 or pG-KJE8. Production of 360 mg/g/h 10S-hydroxy-8(E)-octadecenoic acid from oleic acid
Nostoc punctiforme
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.096
-
alpha-linolenate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
0.236
-
linoleate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
0.345
-
oleate
recombinant enzyme, pH 9.0, 35°C
Nostoc punctiforme
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alpha-linolenate + O2
Nostoc punctiforme
-
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
?
linoleate + O2
Nostoc punctiforme
-
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
?
oleate + O2
Nostoc punctiforme
preferred substrate
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
Nostoc punctiforme
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.096
-
purified recombinant enzyme, substrate alpha-linolenate, pH 9.0, 35°C
Nostoc punctiforme
0.236
-
purified recombinant enzyme, substrate linoleate, pH 9.0, 35°C
Nostoc punctiforme
0.345
-
purified recombinant enzyme, substrate oleate, pH 9.0, 35°C
Nostoc punctiforme
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
alpha-linolenate + O2
-
744582
Nostoc punctiforme
(8E,10S,12Z,15Z)-10-hydroperoxyoctadeca-8,12,15-trienoate
-
-
-
?
linoleate + O2
-
744582
Nostoc punctiforme
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoate
-
-
-
?
additional information
optimization of assay conditions, overview
744582
Nostoc punctiforme
?
-
-
-
-
oleate + O2
preferred substrate
744582
Nostoc punctiforme
(8E,10S)-10-hydroperoxyoctadeca-8-enoate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 63000, recombinant His-tagged enzyme, SDS-PAGE
Nostoc punctiforme
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
recombinant His-tagged enzyme
Nostoc punctiforme
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
recombinant His-tagged enzyme
Nostoc punctiforme
Other publictions for EC 1.13.11.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744582
Kim
Production of 10S-hydroxy-8(E ...
Nostoc punctiforme
Biotechnol. Lett.
39
133-139
2017
1
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1
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1
-
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3
-
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3
-
4
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1
-
-
-
3
-
4
1
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1
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1
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-
-
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1
-
1
-
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1
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-
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3
-
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3
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-
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1
-
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3
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
744396
Mashhadi
Robust inhibitory effects of ...
Nostoc punctiforme
Biochim. Biophys. Acta
1851
1346-1352
2015
-
-
-
-
-
-
11
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
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-
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-
-
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11
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1
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-
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
745297
Brash
An ancient relative of cycloo ...
Nostoc punctiforme
J. Biol. Chem.
289
13101-13111
2014
-
-
1
-
-
-
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3
-
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3
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6
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1
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6
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6
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3
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1
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3
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3
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1
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-
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6
-
-
-
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3
-
-
-
-
-
3
3
-
3
3
725415
Martinez
Biochemical characterization o ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa 42A2
J. Biol. Chem.
285
9339-9345
2010
-
-
-
-
-
-
-
-
-
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1
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4
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-
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2
-
2
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1
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2
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-
-
-
-
-
-
-
-
-
-
-
-
723962
Busquets
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. 42A2
Antonie van Leeuwenhoek
85
129-139
2004
-
-
-
-
-
-
7
3
1
3
1
4
-
2
-
-
1
-
-
-
-
1
13
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
3
1
3
1
4
-
-
-
1
-
-
-
1
13
1
1
-
1
-
1
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