Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,4-Trihydroxybenzene | noncompetitive inhibition | Pseudomonas sp. | |
2,2'-dipyridyl | complete inhibition at 0.5 mM | Pseudomonas sp. | |
3-chlorocatechol | competitive inhibition | Pseudomonas sp. | |
3-fluorocatechol | noncompetitive inhibition | Pseudomonas sp. | |
3-methylcatechol | noncompetitive inhibition | Pseudomonas sp. | |
4-aminoresorcinol | noncompetitive inhibition | Pseudomonas sp. | |
4-Chlorocatechol | competitive inhibition | Pseudomonas sp. | |
4-methylcatechol | uncompetitive inhibition | Pseudomonas sp. | |
5,5'-dithiobis-(2-nitrobenzoic acid) | complete inhibition at 0.5 mM | Pseudomonas sp. | |
AgNO3 | 6% residual activity at 0.5 mM | Pseudomonas sp. | |
catechol | noncompetitive inhibition | Pseudomonas sp. | |
CuSO4 | complete inhibition at 0.1 mM | Pseudomonas sp. | |
EDTA | complete inhibition at 0.5 mM | Pseudomonas sp. | |
FeCl3 | complete inhibition at 0.5 mM | Pseudomonas sp. | |
FeSO4(NH4)2SO4 | 78% residual activity at 0.5 mM | Pseudomonas sp. | |
HgCl2 | 12% residual activity at 0.5 mM | Pseudomonas sp. | |
iodoacetate | 40% residual activity at 0.5 mM | Pseudomonas sp. | |
K3Fe(CN)6 | 14% residual activity at 0.5 mM | Pseudomonas sp. | |
MgSO4 | 79% residual activity at 0.5 mM | Pseudomonas sp. | |
MnCl2 | 53% residual activity at 0.5 mM | Pseudomonas sp. | |
additional information | not inhibited by FeSO4 | Pseudomonas sp. | |
N-ethylmaleimide | 83% residual activity at 0.5 mM | Pseudomonas sp. | |
NaN3 | complete inhibition at 0.5 mM | Pseudomonas sp. | |
o-phenanthroline | 26% residual activity at 0.5 mM | Pseudomonas sp. | |
p-chloromercuribenzoic acid | 65% residual activity at 0.5 mM | Pseudomonas sp. | |
pyrogallol | noncompetitive inhibition | Pseudomonas sp. | |
Tiron | complete inhibition at 0.5 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0467 | - |
2-Aminophenol | pH and temperature not specified in the publication | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains 0.98 mol of Fe2+ per mol of protein | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 40000 + 2 * 32000, SDS-PAGE | Pseudomonas sp. |
40000 | - |
2 * 40000 + 2 * 32000, SDS-PAGE | Pseudomonas sp. |
140000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. Ap-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation, DE52-cellulose column chromatography, DEAE-cellulofine A-500 column chromatography, and gel filtration | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:2-aminophenol-grown cell | - |
Pseudomonas sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4,5-dimethylphenol + O2 | 1.3% activity compared to 2-aminophenol | Pseudomonas sp. | ? | - |
? | |
2-amino-4,5-dimethylphenol + O2 | 1.3% activity compared to 2-aminophenol | Pseudomonas sp. Ap-3 | ? | - |
? | |
2-amino-4-chlorophenol + O2 | 6.8% activity compared to 2-aminophenol | Pseudomonas sp. | ? | - |
? | |
2-amino-4-chlorophenol + O2 | 6.8% activity compared to 2-aminophenol | Pseudomonas sp. Ap-3 | ? | - |
? | |
2-amino-m-cresol + O2 | 4.4% activity compared to 2-aminophenol | Pseudomonas sp. | ? | - |
? | |
2-amino-p-cresol + O2 | 10% activity compared to 2-aminophenol | Pseudomonas sp. | ? | - |
? | |
2-amino-p-cresol + O2 | 10% activity compared to 2-aminophenol | Pseudomonas sp. Ap-3 | ? | - |
? | |
2-aminophenol + O2 | 100% activity | Pseudomonas sp. | 2-aminomuconate 6-semialdehyde | - |
? | |
2-aminophenol + O2 | 100% activity | Pseudomonas sp. Ap-3 | 2-aminomuconate 6-semialdehyde | - |
? | |
6-amino-m-cresol + O2 | 19.1% activity compared to 2-aminophenol | Pseudomonas sp. | ? | - |
? | |
additional information | no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid | Pseudomonas sp. | ? | - |
? | |
additional information | no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid | Pseudomonas sp. Ap-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 40000 + 2 * 32000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
amnA | subunit alpha | Pseudomonas sp. |
amnB | subunit beta | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
3-fluorocatechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0067 | - |
3-chlorocatechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0068 | - |
3-methylcatechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0084 | - |
pyrogallol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0091 | - |
4-aminoresorcinol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0095 | - |
4-methylcatechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0104 | - |
catechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.0104 | - |
4-Chlorocatechol | pH and temperature not specified in the publication | Pseudomonas sp. | |
0.063 | - |
1,2,4-Trihydroxybenzene | pH and temperature not specified in the publication | Pseudomonas sp. |