Literature summary for 1.13.11.74 extracted from

  • Takenaka, S.; Murakami, S.; Shinke, R.; Hatakeyama, K.; Yukawa, H.; Aoki, K.
    Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3 growing on 2-aminophenol and catalytic properties of the purified enzyme (1997), J. Biol. Chem., 272, 14727-14732.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,2,4-Trihydroxybenzene noncompetitive inhibition Pseudomonas sp.
2,2'-dipyridyl complete inhibition at 0.5 mM Pseudomonas sp.
3-chlorocatechol competitive inhibition Pseudomonas sp.
3-fluorocatechol noncompetitive inhibition Pseudomonas sp.
3-methylcatechol noncompetitive inhibition Pseudomonas sp.
4-aminoresorcinol noncompetitive inhibition Pseudomonas sp.
4-Chlorocatechol competitive inhibition Pseudomonas sp.
4-methylcatechol uncompetitive inhibition Pseudomonas sp.
5,5'-dithiobis-(2-nitrobenzoic acid) complete inhibition at 0.5 mM Pseudomonas sp.
AgNO3 6% residual activity at 0.5 mM Pseudomonas sp.
catechol noncompetitive inhibition Pseudomonas sp.
CuSO4 complete inhibition at 0.1 mM Pseudomonas sp.
EDTA complete inhibition at 0.5 mM Pseudomonas sp.
FeCl3 complete inhibition at 0.5 mM Pseudomonas sp.
FeSO4(NH4)2SO4 78% residual activity at 0.5 mM Pseudomonas sp.
HgCl2 12% residual activity at 0.5 mM Pseudomonas sp.
iodoacetate 40% residual activity at 0.5 mM Pseudomonas sp.
K3Fe(CN)6 14% residual activity at 0.5 mM Pseudomonas sp.
MgSO4 79% residual activity at 0.5 mM Pseudomonas sp.
MnCl2 53% residual activity at 0.5 mM Pseudomonas sp.
additional information not inhibited by FeSO4 Pseudomonas sp.
NaN3 complete inhibition at 0.5 mM Pseudomonas sp.
N-ethylmaleimide 83% residual activity at 0.5 mM Pseudomonas sp.
o-phenanthroline 26% residual activity at 0.5 mM Pseudomonas sp.
p-chloromercuribenzoic acid 65% residual activity at 0.5 mM Pseudomonas sp.
pyrogallol noncompetitive inhibition Pseudomonas sp.
Tiron complete inhibition at 0.5 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0467
-
2-Aminophenol pH and temperature not specified in the publication Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains 0.98 mol of Fe2+ per mol of protein Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000 + 2 * 32000, SDS-PAGE Pseudomonas sp.
140000
-
gel filtration Pseudomonas sp.
32000
-
2 * 40000 + 2 * 32000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. Ap-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, DE52-cellulose column chromatography, DEAE-cellulofine A-500 column chromatography, and gel filtration Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:2-aminophenol-grown cell
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid Pseudomonas sp. ?
-
?
additional information no activity with phenol, o-, m-, or p-cresol, aniline, o-, m-, or p-aminobenzoic acid, o-, m-, or p-phenylenediamine, protocatechuic acid, 2,3-dihydroxybenzoic acid, 3-aminophenol, 4-aminophenol, 2-amino-4-isopropylphenol, 2-amino-tert-butylphenol, 3-aminophenazin-2-ol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 3-amino-4-hydroxybenzoic acid, or 4-amino-3-hydroxybenzoic acid, amidol and 3-hydroxyanthralinic acid Pseudomonas sp. Ap-3 ?
-
?
2-amino-p-cresol + O2 10% activity compared to 2-aminophenol Pseudomonas sp. ?
-
?
2-amino-p-cresol + O2 10% activity compared to 2-aminophenol Pseudomonas sp. Ap-3 ?
-
?
2-aminophenol + O2 100% activity Pseudomonas sp. 2-aminomuconate 6-semialdehyde
-
?
2-aminophenol + O2 100% activity Pseudomonas sp. Ap-3 2-aminomuconate 6-semialdehyde
-
?
2-amino-4,5-dimethylphenol + O2 1.3% activity compared to 2-aminophenol Pseudomonas sp. ?
-
?
2-amino-4,5-dimethylphenol + O2 1.3% activity compared to 2-aminophenol Pseudomonas sp. Ap-3 ?
-
?
2-amino-4-chlorophenol + O2 6.8% activity compared to 2-aminophenol Pseudomonas sp. ?
-
?
2-amino-4-chlorophenol + O2 6.8% activity compared to 2-aminophenol Pseudomonas sp. Ap-3 ?
-
?
2-amino-m-cresol + O2 4.4% activity compared to 2-aminophenol Pseudomonas sp. ?
-
?
6-amino-m-cresol + O2 19.1% activity compared to 2-aminophenol Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 40000 + 2 * 32000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
amnA subunit alpha Pseudomonas sp.
amnB subunit beta Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0054
-
3-fluorocatechol pH and temperature not specified in the publication Pseudomonas sp.
0.0104
-
catechol pH and temperature not specified in the publication Pseudomonas sp.
0.0104
-
4-Chlorocatechol pH and temperature not specified in the publication Pseudomonas sp.
0.063
-
1,2,4-Trihydroxybenzene pH and temperature not specified in the publication Pseudomonas sp.
0.0095
-
4-methylcatechol pH and temperature not specified in the publication Pseudomonas sp.
0.0084
-
pyrogallol pH and temperature not specified in the publication Pseudomonas sp.
0.0091
-
4-aminoresorcinol pH and temperature not specified in the publication Pseudomonas sp.
0.0068
-
3-methylcatechol pH and temperature not specified in the publication Pseudomonas sp.
0.0067
-
3-chlorocatechol pH and temperature not specified in the publication Pseudomonas sp.