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Literature summary for 1.13.11.6 extracted from

  • Nandi, D.; Lightcap, E.S.; Koo, Y.K.; Lu, X.; Quancard, J.; Silverman, R.B.
    Purification and inactivation of 3-hydroxyanthranilic acid 3,4-dioxygenase from beef liver (2003), Int. J. Biochem. Cell Biol., 35, 1085-1097.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-chloro-3-hydroxyanthranilic acid 5-20 mM, loss of enzymatic activity as a function of the inhibitor concentration Bos taurus
anthranilic acid competitive inhibition Bos taurus
quinolinic acid competitive inhibition Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16 19 3-Hydroxyanthranilate
-
Bos taurus
36 57 3-hydroxy-4-methylanthranilic acid
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32520
-
PAGE in nondenaturing conditions, pI 4.98 enzyme Bos taurus
32570
-
PAGE in nondenaturing conditions, pI 5.6 enzyme Bos taurus
33000
-
SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column Bos taurus
33000 34000 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyanthranilate + O2 Bos taurus
-
2-amino-3-carboxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
succesive size exclusion and affinity columns Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
115.7
-
last purification step Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-4-methylanthranilic acid + O2
-
Bos taurus ?
-
?
3-hydroxyanthranilate + O2
-
Bos taurus 2-amino-3-carboxymuconate semialdehyde
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33000-34000, gel filtration Bos taurus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
-
4 stable for a month, in Tris-maleate buffer, pH 6.5 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.4
-
Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8
-
quinolinic acid for the pI 5.6 enzyme Bos taurus
2
-
anthranilic acid for the pI 4.98 enzyme Bos taurus
4.9
-
anthranilic acid for the pI 4.98 enzyme Bos taurus
6.5
-
anthranilic acid for the pI 5.6 enzyme Bos taurus
12
-
6-chloro-3-hydroxyanthranilic acid
-
Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
4.98
Bos taurus chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
5.6