KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene | isozyme I | Sphingomonas paucimobilis | |
0.11 | - |
O2 | isozyme I | Sphingomonas paucimobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Sphingomonas paucimobilis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 1 g-atom per mol enzyme | Sphingomonas paucimobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
alpha2, 2 * 52000, SDS-PAGE, isozyme I | Sphingomonas paucimobilis |
94000 | - |
gel filtration, isozyme I, II, III | Sphingomonas paucimobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
stilbene + O2 | Sphingomonas paucimobilis | reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin | ? | - |
? | |
stilbene + O2 | Sphingomonas paucimobilis TMY 1009 | reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
formerly Pseudomonas sp. | - |
Sphingomonas paucimobilis | - |
four isoenzymes | - |
Sphingomonas paucimobilis TMY 1009 | - |
four isoenzymes | - |
Purification (Comment) | Organism |
---|---|
major isozyme I, 4 isozymes by ion-exchange chromatography | Sphingomonas paucimobilis |
Storage Stability | Organism |
---|---|
4°C, one week, 70% activity, isozyme I | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2 | stilbene-type intermediate of diarylpropane-type lignin model compound | Sphingomonas paucimobilis | vanillin | - |
? | |
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2 | stilbene-type intermediate of diarylpropane-type lignin model compound | Sphingomonas paucimobilis TMY 1009 | vanillin | - |
? | |
additional information | catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol | Sphingomonas paucimobilis | ? | - |
? | |
additional information | catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol | Sphingomonas paucimobilis TMY 1009 | ? | - |
? | |
stilbene + O2 | reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin | Sphingomonas paucimobilis | ? | - |
? | |
stilbene + O2 | reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin | Sphingomonas paucimobilis TMY 1009 | ? | - |
? | |
trans-4-hydroxystilbene + O2 | oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation | Sphingomonas paucimobilis | ? | - |
? | |
trans-4-hydroxystilbene + O2 | oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation | Sphingomonas paucimobilis TMY 1009 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | alpha2, 2 * 52000, SDS-PAGE, isozyme I | Sphingomonas paucimobilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
after 3 h, 100% activity retained, isozyme 1 | Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
isozyme I | Sphingomonas paucimobilis |