Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichlorophenylacetate | 5 mM, 50% inhibition | Klebsiella pneumoniae | |
3,4-dihydroxybenzoate | 1 mM, 75% inhibition | Klebsiella pneumoniae | |
Cu2+ | 0.01 mM, 80% inhibition | Klebsiella pneumoniae | |
H2O2 | 10 mM; immediate loss of activity | Klebsiella pneumoniae | |
succinate semialdehyde | 5 mM 65% inhibition | Klebsiella pneumoniae | |
Zn2+ | 0.01 mM, complete inhibition | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
3,4-dihydroxybenzoic acid | - |
Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | less than 0.01 gatom Fe2+ per mol of enzyme | Klebsiella pneumoniae | |
Mg2+ | 1.1-1.3 gatom of Mg2+ per mol of enzyme, essential for activity | Klebsiella pneumoniae | |
Mn2+ | 0.01-0.3 gatom of Mn2+ per mol of enzyme | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
4 * 25500, SDS-PAGE | Klebsiella pneumoniae |
102000 | - |
gel filtration | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
102 | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxybenzoic acid + O2 | - |
Klebsiella pneumoniae | 2-hydroxy-5-carboxymuconate semialdehyde | - |
? | |
3,4-dihydroxyphenylacetate + O2 | - |
Klebsiella pneumoniae | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25500, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
pH 7.5, 0.1 M sodium potassium phosphate, 1 mM DTT, complete loss of activity after 48 h | Klebsiella pneumoniae |
40 | - |
half-life: 13 min | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximal stability at | Klebsiella pneumoniae |