Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | CD I and CD II respectively | Frateuria sp. | |
Fe3+ | CD I and CD II respectively | Frateuria sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | CD I only | Frateuria sp. | |
Hg2+ | CD I only | Frateuria sp. | |
iodoacetic acid | CD I only | Frateuria sp. | |
o-phenanthroline | CD I and CD II respectively | Frateuria sp. | |
p-chloromercuribenzoate | CD I only | Frateuria sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1.2 gatom per mol enzyme protein based on a molecular weight of 38000 and 36000 for CD I and CD II respecetively | Frateuria sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
CD II: 4 * 36000, SDS-PAGE | Frateuria sp. |
37000 | - |
both CD I and CD II, buffer containing NaCl, gel filtration | Frateuria sp. |
38000 | - |
CD I: 4 * 38000, SDS-PAGE | Frateuria sp. |
140000 | - |
buffer omitting NaCl, gel filtration | Frateuria sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Frateuria sp. | - |
ANA-18 | - |
Frateuria sp. ANA-18 | - |
ANA-18 | - |
Purification (Comment) | Organism |
---|---|
CD I and CD II respectively | Frateuria sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Frateuria sp. |
Storage Stability | Organism |
---|---|
6°C, buffer containing 0.1 M NaCl, 50 days without decrease of activity | Frateuria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | poor substrate | Frateuria sp. | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
3-methylcatechol + O2 | poor substrate | Frateuria sp. ANA-18 | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | poor substrate | Frateuria sp. | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | poor substrate | Frateuria sp. ANA-18 | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | - |
Frateuria sp. | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Frateuria sp. ANA-18 | cis,cis-muconate | - |
? | |
additional information | ratios of intradiol to extradiol activity of CD I and CD II 1000:120 and 100:3 respectively | Frateuria sp. | ? | - |
? | |
additional information | ratios of intradiol to extradiol activity of CD I and CD II 1000:120 and 100:3 respectively | Frateuria sp. ANA-18 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | CD I: 4 * 38000, SDS-PAGE | Frateuria sp. |
tetramer | CD II: 4 * 36000, SDS-PAGE | Frateuria sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
for 10 min, stable activity of CD I, activity of CD II decrease to 89% | Frateuria sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
for CD I and CD II respectively | Frateuria sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | for CD I and CD II respectively | Frateuria sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | 5°C, 24 hours | Frateuria sp. |