BRENDA - Enzyme Database
show all sequences of 1.12.98.4

Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction

Ma, K.; Weiss, R.; Adams, M.W.W.; J. Bacteriol. 182, 1864-1871 (2000)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
320000
-
gel filtration
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8
subunits alpha, beta, gamma and delta
-
Purification (Commentary)
Commentary
Organism
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + benzyl viologen
highest activity
439641
Pyrococcus furiosus
H2S + reduced benzyl viologen
-
-
-
?
H2 + methyl viologen
-
439641
Pyrococcus furiosus
H2S + reduced methyl viologen
-
-
-
?
H2 + polysulfide(n)
-
439641
Pyrococcus furiosus
H2S + polysulfide(n-1)
-
-
-
r
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
NADPH + H+ + polysulfide(n)
-
439641
Pyrococcus furiosus
NADP+ + polysulfide(n-1)
-
-
-
r
Subunits
Subunits
Commentary
Organism
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
NADH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
NADPH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
[2Fe-2S]-center
contains 1 [2Fe-2S] center
Pyrococcus furiosus
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
contains 0.83 FAD per mol enzyme
Pyrococcus furiosus
NADH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
NADPH
NADPH and NADH are equally efficient as electron donors for H2 production
Pyrococcus furiosus
[2Fe-2S]-center
contains 1 [2Fe-2S] center
Pyrococcus furiosus
[4Fe-4S]-center
contains 2 [4Fe-4S]-centers
Pyrococcus furiosus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Pyrococcus furiosus
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
contains 21 atoms iron per mol enzyme
Pyrococcus furiosus
Ni2+
contains 0.9 atoms nickel per mol enzyme
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
30000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
39000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
52000
-
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
320000
-
gel filtration
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
H2 + benzyl viologen
highest activity
439641
Pyrococcus furiosus
H2S + reduced benzyl viologen
-
-
-
?
H2 + methyl viologen
-
439641
Pyrococcus furiosus
H2S + reduced methyl viologen
-
-
-
?
H2 + polysulfide(n)
-
439641
Pyrococcus furiosus
H2S + polysulfide(n-1)
-
-
-
r
additional information
ferredoxin from Pyrococcus furiosus is not an efficient electron carrier
439641
Pyrococcus furiosus
?
-
-
-
-
NADPH + H+ + polysulfide(n)
-
439641
Pyrococcus furiosus
NADP+ + polysulfide(n-1)
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE
Pyrococcus furiosus
Other publictions for EC 1.12.98.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716255
Yang
SurR regulates hydrogen produc ...
Pyrococcus furiosus
Mol. Microbiol.
77
1111-1122
2010
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1
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1
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674514
Guiral
A membrane-bound multienzyme, ...
Aquifex aeolicus
J. Biol. Chem.
280
42004-42015
2005
1
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1
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1
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4
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659878
Laska
Membrane-bound hydrogenase and ...
Acidianus ambivalens
Microbiology
149
2357-2371
2003
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1
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3
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5
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1
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1
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1
1
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437780
Keller
Hydrogen-sulfur oxidoreductase ...
Pyrodictium abyssi
Methods Enzymol.
331
442-451
2001
1
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4
1
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1
1
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1
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1
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1
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437781
Laska
Improved purification of the m ...
Acidianus ambivalens
J. Chromatogr. B
737
151-160
2000
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437782
Ng
Purification and some properti ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans NASF-1
J. Biosci. Bioeng.
90
199-203
2000
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1
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6
1
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439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
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1
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5
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4
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1
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1
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437773
Klimmek
The single cysteine residue of ...
Wolinella succinogenes
Eur. J. Biochem.
263
79-84
1999
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1
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1
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4
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745391
Silva
On the prosthetic groups of t ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
4
284-291
1999
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745603
Selvaggi
-
In vitro hydrogen photoproduc ...
Pyrococcus furiosus
J. Photochem. Photobiol. A
125
107-112
1999
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1
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437778
Dirmeier
Purification and properties of ...
Pyrodictium abyssi
Eur. J. Biochem.
252
486-491
1998
1
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437779
Sugio
Purification and characterizat ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
62
705-709
1998
2
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7
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675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
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437776
Arendsen
Redox properties of the sulfhy ...
Pyrococcus furiosus
FEBS Lett.
368
117-121
1995
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723118
Pedroni
Characterization of the locus ...
Pyrococcus furiosus
Microbiology
141
449-458
1995
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437774
Fauque
-
Sulfur reductases from spirill ...
Sulfurospirillum deleyianum, Wolinella succinogenes
Methods Enzymol.
243
367-383
1994
3
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437775
Jankielewicz
-
Polysulfide reductase and form ...
Wolinella succinogenes
Arch. Microbiol.
162
238-242
1994
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437777
Childers
Characterization and regulatio ...
Thermotoga neapolitana
Appl. Environ. Microbiol.
60
2622-2626
1994
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722289
Ma
-
Hydrogen production from pyruv ...
Pyrococcus furiosus
FEMS Microbiol. Lett.
122
245-250
1994
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439664
Ma
Hydrogenase of the hyperthermo ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
90
5341-5344
1993
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Sulfurospirillum deleyianum
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2
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2
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2
1
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1
2
1
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1
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4
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2
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2
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1
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1
2
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1
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4
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437770
Zöphel
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Investigations on microbial su ...
Desulfomicrobium baculatum, Desulfomicrobium baculatum 9974, Desulfuromonas acetexigens, Desulfuromonas acetexigens 2873, Desulfuromonas acetoxidans, Desulfuromonas acetoxidans 5071, Desulfuromusa succinoxidans, Desulfuromusa succinoxidans Go20, Sulfurospirillum deleyianum
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4
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7
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10
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1
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10
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1
1
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5
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16
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1
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4
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7
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10
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1
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1
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5
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16
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1
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437771
Schröder
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Isolation of the sulphur reduc ...
Wolinella succinogenes
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1
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3
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2
1
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1
1
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1
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1
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3
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2
1
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1
1
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1
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1
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1
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