BRENDA - Enzyme Database
show all sequences of 1.12.98.4

Investigations on microbial sulfur respiration. Isolation, purification, and characterization of cellular components from Spirillum 5175

Zöphel, A.; Kennedy, M.C.; Beinert, H.; Kroneck, P.M.H.; Eur. J. Biochem. 195, 849-856 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
anthraquinone-2-sulfonate
activation, in vitro electron donor
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Sulfurospirillum deleyianum
General Stability
General Stability
Organism
glycerol does not stabilize during purification
Sulfurospirillum deleyianum
PMSF does not stabilize during purification
Sulfurospirillum deleyianum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
no activity
Sulfurospirillum deleyianum
5737
-
membrane
-
Sulfurospirillum deleyianum
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
iron-sulfur (non-heme) protein; low temperature EPR investigations
Sulfurospirillum deleyianum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur
Sulfurospirillum deleyianum
constitutive membrane-bound enzyme
H2S
-
Sulfurospirillum deleyianum
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
additional information
octyl-2-D-glucopyranoside, 78% of activity
Sulfurospirillum deleyianum
Triton X-100
68,5% of activity
Sulfurospirillum deleyianum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfurospirillum deleyianum
-
-
-
Purification (Commentary)
Commentary
Organism
hydroxyapatite chromatography; purified with 2-octylglucoside, not perchlorate or urea
Sulfurospirillum deleyianum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
sulfur can be reduced by photochemical reduction in the presence of phenosafranin
437772
Sulfurospirillum deleyianum
?
-
-
-
-
organic trisulfides + electron donor
i.e. (R-S-S-S-R)2, R: CH2CH2NH2
437772
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
-
-
?
sulfur
constitutive membrane-bound enzyme
437772
Sulfurospirillum deleyianum
H2S
-
437772
Sulfurospirillum deleyianum
?
sulfur + electron donor
-
437772
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437772
Sulfurospirillum deleyianum
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
anthraquinone-2-sulfonate
activation, in vitro electron donor
Sulfurospirillum deleyianum
additional information
no cytochrome c3, c7 or b involved
Sulfurospirillum deleyianum
General Stability (protein specific)
General Stability
Organism
glycerol does not stabilize during purification
Sulfurospirillum deleyianum
PMSF does not stabilize during purification
Sulfurospirillum deleyianum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
no activity
Sulfurospirillum deleyianum
5737
-
membrane
-
Sulfurospirillum deleyianum
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
iron-sulfur (non-heme) protein; low temperature EPR investigations
Sulfurospirillum deleyianum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sulfur
Sulfurospirillum deleyianum
constitutive membrane-bound enzyme
H2S
-
Sulfurospirillum deleyianum
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
additional information
octyl-2-D-glucopyranoside, 78% of activity
Sulfurospirillum deleyianum
Triton X-100
68,5% of activity
Sulfurospirillum deleyianum
Purification (Commentary) (protein specific)
Commentary
Organism
hydroxyapatite chromatography; purified with 2-octylglucoside, not perchlorate or urea
Sulfurospirillum deleyianum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
sulfur can be reduced by photochemical reduction in the presence of phenosafranin
437772
Sulfurospirillum deleyianum
?
-
-
-
-
organic trisulfides + electron donor
i.e. (R-S-S-S-R)2, R: CH2CH2NH2
437772
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
-
-
?
sulfur
constitutive membrane-bound enzyme
437772
Sulfurospirillum deleyianum
H2S
-
437772
Sulfurospirillum deleyianum
?
sulfur + electron donor
-
437772
Sulfurospirillum deleyianum
H2S + oxidized electron donor
-
437772
Sulfurospirillum deleyianum
?
Other publictions for EC 1.12.98.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716255
Yang
SurR regulates hydrogen produc ...
Pyrococcus furiosus
Mol. Microbiol.
77
1111-1122
2010
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1
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1
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674514
Guiral
A membrane-bound multienzyme, ...
Aquifex aeolicus
J. Biol. Chem.
280
42004-42015
2005
1
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4
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659878
Laska
Membrane-bound hydrogenase and ...
Acidianus ambivalens
Microbiology
149
2357-2371
2003
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1
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3
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5
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1
1
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437780
Keller
Hydrogen-sulfur oxidoreductase ...
Pyrodictium abyssi
Methods Enzymol.
331
442-451
2001
1
-
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4
1
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1
1
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1
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4
1
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1
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1
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1
1
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1
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1
1
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437781
Laska
Improved purification of the m ...
Acidianus ambivalens
J. Chromatogr. B
737
151-160
2000
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1
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1
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437782
Ng
Purification and some properti ...
Acidithiobacillus ferrooxidans, Acidithiobacillus ferrooxidans NASF-1
J. Biosci. Bioeng.
90
199-203
2000
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1
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6
1
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1
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439641
Ma
Characterization of hydrogenas ...
Pyrococcus furiosus
J. Bacteriol.
182
1864-1871
2000
-
-
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-
1
2
5
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4
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1
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5
1
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5
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5
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5
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5
1
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437773
Klimmek
The single cysteine residue of ...
Wolinella succinogenes
Eur. J. Biochem.
263
79-84
1999
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1
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4
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745391
Silva
On the prosthetic groups of t ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
4
284-291
1999
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2
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745603
Selvaggi
-
In vitro hydrogen photoproduc ...
Pyrococcus furiosus
J. Photochem. Photobiol. A
125
107-112
1999
-
1
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437778
Dirmeier
Purification and properties of ...
Pyrodictium abyssi
Eur. J. Biochem.
252
486-491
1998
1
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1
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3
1
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437779
Sugio
Purification and characterizat ...
thermophilic iron-oxidizing bacterium strain TI-1
Biosci. Biotechnol. Biochem.
62
705-709
1998
2
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7
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1
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4
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675504
Schneider
Crystal structure of dimethyl ...
Rhodobacter capsulatus
J. Mol. Biol.
263
53-69
1996
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1
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437776
Arendsen
Redox properties of the sulfhy ...
Pyrococcus furiosus
FEBS Lett.
368
117-121
1995
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1
2
2
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723118
Pedroni
Characterization of the locus ...
Pyrococcus furiosus
Microbiology
141
449-458
1995
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437774
Fauque
-
Sulfur reductases from spirill ...
Sulfurospirillum deleyianum, Wolinella succinogenes
Methods Enzymol.
243
367-383
1994
3
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437775
Jankielewicz
-
Polysulfide reductase and form ...
Wolinella succinogenes
Arch. Microbiol.
162
238-242
1994
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437777
Childers
Characterization and regulatio ...
Thermotoga neapolitana
Appl. Environ. Microbiol.
60
2622-2626
1994
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722289
Ma
-
Hydrogen production from pyruv ...
Pyrococcus furiosus
FEMS Microbiol. Lett.
122
245-250
1994
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439664
Ma
Hydrogenase of the hyperthermo ...
Pyrococcus furiosus
Proc. Natl. Acad. Sci. USA
90
5341-5344
1993
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437772
Zöphel
Investigations on microbial su ...
Sulfurospirillum deleyianum
Eur. J. Biochem.
195
849-856
1991
2
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2
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2
1
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437770
Zöphel
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Investigations on microbial su ...
Desulfomicrobium baculatum, Desulfomicrobium baculatum 9974, Desulfuromonas acetexigens, Desulfuromonas acetexigens 2873, Desulfuromonas acetoxidans, Desulfuromonas acetoxidans 5071, Desulfuromusa succinoxidans, Desulfuromusa succinoxidans Go20, Sulfurospirillum deleyianum
Arch. Microbiol.
150
72-77
1988
4
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7
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10
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1
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10
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1
1
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5
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16
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1
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4
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7
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10
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1
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1
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5
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16
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1
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437771
Schröder
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Isolation of the sulphur reduc ...
Wolinella succinogenes
Arch. Microbiol.
149
572-579
1988
1
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3
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2
1
2
1
1
1
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1
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1
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1
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1
1
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1
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3
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2
1
2
1
1
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1
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1
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2
1
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1
1
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