Literature summary for 1.12.1.3 extracted from

Wu, C.H.; Ponir, C.A.; Haja, D.K.; Adams, M.W.W.
Improved production of the NiFe-hydrogenase from Pyrococcus furiosus by increased expression of maturation genes (2018), Protein Eng. Des. Sel., 31, 337-344 .

Cloned(Commentary)

Cloned (Comment)	Organism
construction of a strain of Pyrococcus furiosus that contains a second set of the eight genes involved in the maturation of the catalytic subunit and insertion of the [NiFe]-site, along with a second set of the four genes encoding the NADPH-dependent cytoplasmic [NiFe]-hydrogenase (SHI) structural subunits. This results in a 40% higher yield of the purified affinity-tagged enzyme and the content of the inactive trimeric form decreases to 5% of the total protein	Pyrococcus furiosus

Cloned (Comment)

Organism

construction of a strain of Pyrococcus furiosus that contains a second set of the eight genes involved in the maturation of the catalytic subunit and insertion of the [NiFe]-site, along with a second set of the four genes encoding the NADPH-dependent cytoplasmic [NiFe]-hydrogenase (SHI) structural subunits. This results in a 40% higher yield of the purified affinity-tagged enzyme and the content of the inactive trimeric form decreases to 5% of the total protein

Pyrococcus furiosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Pyrococcus furiosus	5737	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + NADP+	Pyrococcus furiosus	-	H+ + NADPH	-	?
H2 + NADP+	Pyrococcus furiosus ATCC 43587	-	H+ + NADPH	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	E7FHU4 AND E7FI44	E7FHU4: sulfhydrogenase 1 subunit delta, E7FI44: sulfhydrogenase 1 subunit alpha	-
Pyrococcus furiosus ATCC 43587	E7FHU4 AND E7FI44	E7FHU4: sulfhydrogenase 1 subunit delta, E7FI44: sulfhydrogenase 1 subunit alpha	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + NADP+	-	Pyrococcus furiosus	H+ + NADPH	-	?
H2 + NADP+	-	Pyrococcus furiosus ATCC 43587	H+ + NADPH	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	-	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
NADPH-dependent [NiFe]-hydrogenase	-	Pyrococcus furiosus
SHI	-	Pyrococcus furiosus