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Literature summary for 1.11.2.3 extracted from

  • Brunetti, S.C.; Arseneault, M.K.M.; Wright, J.A.; Wang, Z.; Ehdaeivand, M.R.; Lowden, M.J.; Rivoal, J.; Khalil, H.B.; Garg, G.; Gulick, P.J.
    The stress induced caleosin, RD20/CLO3, acts as a negative regulator of GPA1 in Arabidopsis (2021), Plant Mol. Biol., 107, 159-175 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaves and in Escherichia coli BL21 (DE3) cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D70A the disruption of calcium-binding in this mutant abolishes the interaction with the heterotrimeric G-protein complex GPA1. The mutant has altered leaf development Arabidopsis thaliana
E81R the disruption of calcium-binding in this mutant abolishes the interaction with the heterotrimeric G-protein complex GPA1. The mutant has altered leaf development Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is a calcium-binding protein Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Wassilewskija
-

Purification (Commentary)

Purification (Comment) Organism
nickel agarose bead chromatography and HiTrap Q FF Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Arabidopsis thaliana
-

Synonyms

Synonyms Comment Organism
caleosin
-
Arabidopsis thaliana
RD20/CLO3
-
Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the enzyme is strongly induced by drought, salt and abscisic acid up

General Information

General Information Comment Organism
physiological function the enzyme interacts with the alpha subunit of the heterotrimeric G-protein complex in Arabidopsis and affects etiolation and leaf morphology. The enzyme plays a key role in the signaling pathway controlling hypocotyl length in dark grown seedlings and in leaf morphology Arabidopsis thaliana