Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.7 extracted from

  • Elsayed, A.; Hegazy, U.; Hegazy, M.; Abdel-Ghany, S.; Salama, W.; Salem, A.; Fahmy, A.
    Purification and biochemical characterization of peroxidase isoenzymes from Ficus carica latex (2018), Biocatal. Agricult. Biotechnol., 16, 1-9 .
No PubMed abstract available
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM, 17% loss of activity, isoenzyme FP2; 10 mM, 42% loss of activity, isoenzyme FP1; 10 mM, 53% loss of activity, isoenzyme FP3 Ficus carica
Cu2+ 10 mM, 67% loss of activity, isoenzyme FP3; 10 mM, complete loss of activity, isoenzyme FP1 Ficus carica
EDTA 10 mM, 14% loss of activity, isoenzyme FP2; 10 mM, 36% loss of activity, isoenzyme FP1; 10 mM, 88% loss of activity, isoenzyme FP3 Ficus carica
Hg2+ 10 mM, 54% loss of activity, isoenzyme FP2; 10 mM, 82% loss of activity, isoenzyme FP3; 10 mM, 91% loss of activity, isoenzyme FP1 Ficus carica
Mn2+ 10 mM, 19% loss of activity, isoenzyme FP1; 10 mM, 2% loss of activity, isoenzyme FP2; 10 mM, 75% loss of activity, isoenzyme FP3 Ficus carica
NaN3 10 mM, 98% loss of activity, isoenzyme FP2; 10 mM, 99% loss of activity, isoenzyme FP1; 10 mM, complete loss of activity, isoenzyme FP3 Ficus carica
Ni2+ 10 mM, 14% loss of activity, isoenzyme FP1; 10 mM, 27% loss of activity, isoenzyme FP2; 10 mM, 55% loss of activity, isoenzyme FP3 Ficus carica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 H2O2 pH 5.5, temperature not specified in the publication, isoenzyme FP3 Ficus carica
0.25
-
 H2O2 pH 5.5, temperature not specified in the publication, isoenzyme FP2 Ficus carica
0.34
-
 H2O2 pH 5.5, temperature not specified in the publication, isoenzyme FP1 Ficus carica
3.33
-
 o-phenylenediamine pH 5.5, temperature not specified in the publication, isoenzyme FP3 Ficus carica
3.64
-
 o-phenylenediamine pH 5.5, temperature not specified in the publication, isoenzyme FP2 Ficus carica
3.87
-
 o-phenylenediamine pH 5.5, temperature not specified in the publication, isoenzyme FP1 Ficus carica
5.68
-
 guaiacol pH 5.5, temperature not specified in the publication, isoenzyme FP1 Ficus carica
6.89
-
 guaiacol pH 5.5, temperature not specified in the publication, isoenzyme FP2 Ficus carica
15.94
-
 guaiacol pH 5.5, temperature not specified in the publication, isoenzyme FP3 Ficus carica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, activity of both FP1 and FP2 peroxidase isoenzymes is increased Ficus carica
Cu2+ 10 mM, slight activation of isoenzyme FP2, 3.7fold activation of isoenzyme FP3 Ficus carica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration, isoenzyme FP1 Ficus carica

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10%, 25% loss of activity, isoenzyme FP1 Ficus carica
Acetone 10%, 42% loss of activity, isoenzyme FP2 Ficus carica
Acetone 10%, 92% loss of activity, isoenzyme FP3 Ficus carica
DMSO 10%, 48% loss of activity, isoenzyme FP1 Ficus carica
DMSO 10%, 54% loss of activity, isoenzyme FP2 Ficus carica
DMSO 10%, 90% loss of activity, isoenzyme FP3 Ficus carica
Ethanol 10%, 33% loss of activity, isoenzyme FP1 Ficus carica
Ethanol 10%, 36% loss of activity, isoenzyme FP3 Ficus carica
Ethanol 10%, 55% loss of activity, isoenzyme FP2 Ficus carica
Methanol 10%, 30% loss of activity, isoenzyme FP1 Ficus carica
Methanol 10%, 36% loss of activity, isoenzyme FP2 Ficus carica
Methanol 10%, 42% loss of activity, isoenzyme FP3 Ficus carica

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ficus carica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Ficus carica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aminoantipyrine + H2O2 1.5% of the activity with o-phenylenediamine, isoenzyme FP2 Ficus carica ? + H2O
-
 ?
aminoantipyrine + H2O2 2.5% of the activity with o-phenylenediamine, isoenzyme FP1 Ficus carica ? + H2O
-
 ?
aminoantipyrine + H2O2 7.2% of the activity with o-phenylenediamine, isoenzyme FP3 Ficus carica ? + H2O
-
 ?
guaiacol + H2O2 17% of the activity with o-phenylenediamine, isoenzyme FP1 Ficus carica tetraguaiacol + H2O
-
 ?
guaiacol + H2O2 3.7% of the activity with o-phenylenediamine, isoenzyme FP2 Ficus carica ? + H2O
-
 ?
guaiacol + H2O2 6.8% of the activity with o-phenylenediamine, isoenzyme FP3 Ficus carica ? + H2O
-
 ?
o-dianisidine + H2O2 10% of the activity with o-phenylenediamine, isoenzyme FP2 Ficus carica ? + H2O
-
 ?
o-dianisidine + H2O2 11% of the activity with o-phenylenediamine, isoenzyme FP3 Ficus carica ? + H2O
-
 ?
o-dianisidine + H2O2 30% of the activity with o-phenylenediamine, isoenzyme FP1 Ficus carica ? + H2O
-
 ?
o-phenylenediamine + H2O2
-
 Ficus carica ? + H2O
-
 ?
pyrogallol + H2O2 3.8% of the activity with o-phenylenediamine, isoenzyme FP2 Ficus carica ? + H2O
-
 ?
pyrogallol + H2O2 4.5% of the activity with o-phenylenediamine, isoenzyme FP3 Ficus carica ? + H2O
-
 ?
pyrogallol + H2O2 5.3% of the activity with o-phenylenediamine, isoenzyme FP1 Ficus carica ? + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE, isoenzyme FP1 Ficus carica

Synonyms

Synonyms Comment Organism
FP1
-
 Ficus carica
Fp2
-
 Ficus carica
FP3
-
 Ficus carica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 isoenzyme FP2 Ficus carica
40
-
 isoenzyme FP1 and FP3 Ficus carica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 isoenzyme FP3 is stable at Ficus carica
40
-
 isoenzyme FP2 is stable at Ficus carica
50
-
 isoenzyme FP1 is stable at Ficus carica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 isoenzyme FP1 and FP3 Ficus carica
7
-
 isoenzyme FP2 Ficus carica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 FP2 isoenzyme is stable Ficus carica
4.5 5.5 FP3 isoenzyme is stable Ficus carica
5 7.5 FP1 isoenzyme is stable Ficus carica