KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
75 | - |
H2O2 | pH 7.0, 25°C | Psychrobacter piscatorii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, SDS-PAGE | Psychrobacter piscatorii |
247000 | - |
gel filtration | Psychrobacter piscatorii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Psychrobacter piscatorii | - |
O2 + 2 H2O | - |
? | |
2 H2O2 | Psychrobacter piscatorii T-3 | - |
O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter piscatorii | A0A0T6DN91 | isolated from the drain pool of a plant that uses H2O2 as a bleaching agent | - |
Psychrobacter piscatorii T-3 | A0A0T6DN91 | isolated from the drain pool of a plant that uses H2O2 as a bleaching agent | - |
Purification (Comment) | Organism |
---|---|
native enzyme 11fold by anion exchange and hydrophobic chromatography, followed by ultrafiltration | Psychrobacter piscatorii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19700 | - |
native enzyme, cell extract, pH 7.0, 25°C | Psychrobacter piscatorii |
222000 | - |
purified native enzyme, pH 7.0, 25°C | Psychrobacter piscatorii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Psychrobacter piscatorii | O2 + 2 H2O | - |
? | |
2 H2O2 | - |
Psychrobacter piscatorii T-3 | O2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 59000, SDS-PAGE | Psychrobacter piscatorii |
Synonyms | Comment | Organism |
---|---|---|
PktA | - |
Psychrobacter piscatorii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Psychrobacter piscatorii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | broad optimum | Psychrobacter piscatorii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | activity range, 53.4% of maximal activity at pH 4.0, 18.4% at pH 3.0 | Psychrobacter piscatorii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
purified native enzyme, 15 min, 60°C, complete inactivation | Psychrobacter piscatorii |
8 | - |
purified native enzyme, 15 min, 60°C, most stable at | Psychrobacter piscatorii |
11 | - |
purified native enzyme, 15 min, 60°C, complete inactivation | Psychrobacter piscatorii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | binding sites of the distal region of heme with Val106, Thr128 and Phe143, and proximal sites of heme with Tyr348 and Arg355 are well conserved | Psychrobacter piscatorii | |
additional information | the enzyme contains a NADPH-binding site with His184, Arg193, Val292 and Lys295 | Psychrobacter piscatorii |
General Information | Comment | Organism |
---|---|---|
evolution | the dismutation reaction of H2O2 in microorganisms has evolved in three phylogenetically unrelated protein types: monofunctional catalase, catalase-peroxidase and Mn-catalase, phylogenetic analysis, overview. PktA is a clade 3 catalase. The active sites with His65, Ser104, and Asn138, binding sites of the distal region of heme with Val106, Thr128, and Phe143, and proximal sites of heme with Tyr348 and Arg355 are well conserved | Psychrobacter piscatorii |
additional information | the enzyme exhibits an extraordinarily high catalase activity, active sites residues are His65, Ser104, and Asn138 | Psychrobacter piscatorii |