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Literature summary for 1.11.1.24 extracted from

  • Kong, W.; Shiota, S.; Shi, Y.; Nakayama, H.; Nakayama, K.
    A novel peroxiredoxin of the plant Sedum lineare is a homologue of Escherichia coli bacterioferritin co-migratory protein Bcp (2000), Biochem. J., 351, 107-114 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli overexpression system (BL21 DE3)pKD601. Expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo Sedum lineare

Protein Variants

Protein Variants Comment Organism
C44S expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo. Escherichia coli bcp cells producing the mutant enzyme show the same sensitivities to the organic peroxides as those of the control bcp cells Sedum lineare
C49S expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo. Escherichia coli bcp cells producing the mutant enzyme show the same sensitivities to the organic peroxides as those of the control bcp cells. Except that the bcp cells producing C49S show partial resistance to tert-butyl hydroperoxide Sedum lineare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16699
-
 calculated from sequence Sedum lineare
17000
-
 SDS-PAGE Sedum lineare

Organism

Organism UniProt Comment Textmining
Sedum lineare Q9MB35
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes C44S and C49S Sedum lineare

Source Tissue

Source Tissue Comment Organism Textmining
stem leaf
-
 Sedum lineare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thioredoxin + cumene hydroperoxide
-
 Sedum lineare thioredoxin disulfide + H2O + cumene hydroxide
-
 ?
thioredoxin + H2O2
-
 Sedum lineare thioredoxin disulfide + 2 H2O
-
 ?
thioredoxin + tert-butyl hydroperoxide
-
 Sedum lineare thioredoxin disulfide + H2O + tert-butyl alcohol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 17000, SDS-PAGE, the enzyme does not form a homodimer under non-reducing conditions, even after treatment with H2O2 Sedum lineare

Synonyms

Synonyms Comment Organism
PrxQ
-
 Sedum lineare

pI Value

Organism Comment pI Value Maximum pI Value
Sedum lineare calculated from sequence
-
 9.7