Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces wedmorensis |
Protein Variants | Comment | Organism |
---|---|---|
E142A | complete loss of enzymatic activity in vivo and in vitro, 2.1% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105 | Streptomyces wedmorensis |
H138A | inactive in vivo, but in vitro it retains 27% of the active site iron and nearly 20% of the wild-type activity, limited self-hydroxylation (at Tyr105) | Streptomyces wedmorensis |
H180A | complete loss of enzymatic activity in vivo and in vitro, 5.8% of the active site iron as compared to wild-type enzyme, no self-hydroxylation at Tyr105 | Streptomyces wedmorensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | non-heme iron-dependent enzyme | Streptomyces wedmorensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces wedmorensis | Q56185 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxypropylphosphonic acid + 2 NADH + O2 | - |
Streptomyces wedmorensis | cis-(1R,2S)-epoxypropylphosphonic acid + 2 H2O + 2 NAD+ | i.e. fosfomycin | ? |
Synonyms | Comment | Organism |
---|---|---|
fom4 | - |
Streptomyces wedmorensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Streptomyces wedmorensis | |
NADH | - |
Streptomyces wedmorensis |