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Literature summary for 1.11.1.19 extracted from

  • Liu, X.; Yuan, Z.; Wang, J.; Cui, Y.; Liu, S.; Ma, Y.; Gu, L.; Xu, S.
    Crystal structure and biochemical features of dye-decolorizing peroxidase YfeX from Escherichia coli O157 Asp(143) and Arg(232) play divergent roles toward different substrates (2017), Biochem. Biophys. Res. Commun., 484, 40-44 .
    View publication on PubMed
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with heme, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 20% (w/v) PEG3350 and 0.1 M Bis-Tris pH 5.5 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D143A the mutant shows no activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and guaiacol, but about 20% of wild type activity with Reactive Blue 19 and 10% activity with catechol Escherichia coli
D143N the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
H215A the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
L246W the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli
R232E the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 40% of wild type activity with guaiacol and 35% activity with catechol Escherichia coli
R232L the mutant shows no activity towards Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), but about 90% of wild type activity with guaiacol and 50% activity with catechol Escherichia coli
S234Y the mutant shows no activity towards guaiacol, catechol, Reactive Blue 19 and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli O157
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Escherichia coli O157 ?
-
 ?
catechol + H2O2 + H+
-
 Escherichia coli ?
-
 ?
catechol + H2O2 + H+
-
 Escherichia coli O157 ?
-
 ?
guaiacol + H2O2 + H+
-
 Escherichia coli ?
-
 ?
guaiacol + H2O2 + H+
-
 Escherichia coli O157 ?
-
 ?
Reactive Blue 19 + H2O2
-
 Escherichia coli ?
-
 ?
Reactive Blue 19 + H2O2
-
 Escherichia coli O157 ?
-
 ?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Escherichia coli
YfeX
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme
-
 Escherichia coli