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Literature summary for 1.11.1.19 extracted from

  • Li, J.; Liu, C.; Li, B.; Yuan, H.; Yang, J.; Zheng, B.
    Identification and molecular characterization of a novel DyP-type peroxidase from Pseudomonas aeruginosa PKE117 (2012), Appl. Biochem. Biotechnol., 166, 774-785.
    View publication on PubMed
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 10 mM, 33% residual activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.107
-
 Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 10 mM, 117% of initial activity Pseudomonas aeruginosa
Co2+ 10 mM, 115% of initial activity Pseudomonas aeruginosa
Zn2+ 10 mM, 122% of initial activity Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Pseudomonas aeruginosa
64000
-
 gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa D5LRR6
-
-
Pseudomonas aeruginosa PKE117 D5LRR6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2 + H+ 18.3% of the activity with Reactive Blue 5 Pseudomonas aeruginosa ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+ 18.3% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 ?
-
 ?
Reactive Black 5 + H2O2 16.4% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Reactive Black 5 + H2O
-
 ?
Reactive Black 5 + H2O2 16.4% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Reactive Black 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 19% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Blue 19 + H2O2 19% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Reactive Blue 5 + H2O
-
 ?
Reactive Blue 5 + H2O2
-
 Pseudomonas aeruginosa phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
-
 ?
Reactive Blue 5 + H2O2
-
 Pseudomonas aeruginosa PKE117 phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
-
 ?
Vat Blue 6 + H2O2 5.1% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Vat Blue 6 + H2O
-
 ?
Vat Blue 6 + H2O2 5.1% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Vat Blue 6 + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
DyP
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 4 h, 83% residual activity Pseudomonas aeruginosa
70
-
 1 h, more than 70% residual activity Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0235
-
 Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 rapid loss of activity below Pseudomonas aeruginosa
6.5 7 30 min, stable Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
heme enzyme displays typical heme absorbance of Soret band, with an Rz value of 1.18 Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa calculated
-
 4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.219
-
 Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa