Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
E37K/V160A/T184M/Q202L | mutant obtained by directed evolution, increase in activity and temperature stability | Pleurotus eryngii |
additional information | an engineered N-terminally truncated variant of mutant E37K/V160A/T184M/Q202L displays similar biochemical properties to those of the non-truncated counterpart in terms of kinetics, stability and spectroscopic features. Additional cycles of evolution raised the melting temperature by 8 degrees and significantly increased the enzyme's stability at alkaline pHs. In addition, the Km for H2O2 is enhanced up to 15fold while the catalytic efficiency is maintained, and there is an improvement in peroxide stability | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
Reactive Black 5 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.007 | - |
Reactive Black 5 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.045 | - |
Mn2+ | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.051 | - |
H2O2 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.056 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.12 | - |
Mn2+ | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.2 | - |
H2O2 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
0.54 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
6.5 | - |
2,6-dimethoxyphenol | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
32 | - |
2,6-dimethoxyphenol | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35244 | - |
x * 35258, calculated, x * 35244, MALDI-TOF | Pleurotus eryngii |
35258 | - |
x * 35258, calculated, x * 35244, MALDI-TOF | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | isoform Vpl2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | degree of glycosylation is 9% | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2,6-dimethoxyphenol + 2 H2O2 | - |
Pleurotus eryngii | coerulignone + 2 H2O | - |
? | |
2 Mn2+ + 2 H+ + H2O2 | - |
Pleurotus eryngii | 2 Mn3+ + 2 H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus eryngii | ? + H2O | - |
? | |
Reactive Black 5 + H2O2 | - |
Pleurotus eryngii | oxidized Reactive Black 5 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35258, calculated, x * 35244, MALDI-TOF | Pleurotus eryngii |
Synonyms | Comment | Organism |
---|---|---|
Vpl2 | - |
Pleurotus eryngii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
wild-type, melting temperature | Pleurotus eryngii |
59.4 | - |
mutant E37K/V160A/T184M/Q202L, melting temperature | Pleurotus eryngii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
Reactive Black 5 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
11.8 | - |
Reactive Black 5 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
54 | - |
Mn2+ | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
58 | - |
2,6-dimethoxyphenol | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
75 | - |
Mn2+ | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
98 | - |
2,6-dimethoxyphenol | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
135 | - |
H2O2 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
220 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
365 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
490 | - |
H2O2 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
wild-type | Pleurotus eryngii |
3.5 | - |
mutant E37K/V160A/T184M/Q202L | Pleurotus eryngii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
2,6-dimethoxyphenol | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
9.1 | - |
2,6-dimethoxyphenol | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
410 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
630 | - |
Mn2+ | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
1190 | - |
Mn2+ | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
1600 | - |
Reactive Black 5 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
1670 | - |
Reactive Black 5 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
2400 | - |
H2O2 | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
2650 | - |
H2O2 | wild-type, pH 3.5, temperature not specified in the publication | Pleurotus eryngii | |
6480 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | mutant E37K/V160A/T184M/Q202L, pH 3.5, temperature not specified in the publication | Pleurotus eryngii |