Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
K428E | 1.3fold decrease in kcat/Km for the substrate guaiacol | Thermus thermophilus |
K428L | 1.6fold decrease in kcat/Km for the substrate guaiacol. 70% decrease in activity of mutant enzyme after 4 h at 80°C. 30% decrease in activity of wild-type enzyme after 4 h at 80°C | Thermus thermophilus |
K428M | 1.4fold increase in kcat/Km for the substrate guaiacol | Thermus thermophilus |
K428R | 1.3fold decrease in kcat/Km for the substrate guaiacol | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.384 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
0.391 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428M | Thermus thermophilus | |
0.473 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428R | Thermus thermophilus | |
0.52 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428E | Thermus thermophilus | |
0.575 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428L | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus SG0.5JP17-16 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guaiacol + O2 | - |
Thermus thermophilus | ? | - |
? | |
guaiacol + O2 | - |
Thermus thermophilus SG0.5JP17-16 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
wild-type and K428L, K428M, K428R and K428E mutant enzymes | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
0-4 h, residual enzymatic activity decreases with the increase of the incubation time. The wild-type, K428M, K428E and K428R enzymes remain 70% of corresponding initial activity after incubated for 4 h. The K428L mutant is most affected, only 30% of the initial activity is retained after 4 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.7 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428R | Thermus thermophilus | |
5.8 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428L | Thermus thermophilus | |
6.1 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
6.2 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428E | Thermus thermophilus | |
8.4 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428M | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
wild-type and K428L, K428M, K428R and K428E mutant enzymes | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428L | Thermus thermophilus | |
12 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428E | Thermus thermophilus | |
12 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428R | Thermus thermophilus | |
16 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
22 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme K428M | Thermus thermophilus |