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Literature summary for 1.10.3.2 extracted from

  • Aka, B.Z.E.; Djeni, T.N.; Konan, H.K.; Semeniuc, C.A.; Rotar, A.M.; Suharoschi, R.; Dje, M.K.
    Characterization of a potential isozyme laccase from Trametes polyzona MPS1-3 and its contribution to palm oil mill effluent treatment (2021), Curr. Microbiol., 78, 3246-3257 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
hexane 25%, activates to 106.13% of control Trametes polyzona

Inhibitors

Inhibitors Comment Organism Structure
acetone 25%, 20% inhibition Trametes polyzona
butanol 25%, 42% inhibition Trametes polyzona
chloroform 25%, 25% inhibition Trametes polyzona
ethanol 25%, 28% inhibition Trametes polyzona
methanol 25%, 17% inhibition Trametes polyzona
Mg2+ 2.5 mM 9% inhibition Trametes polyzona
additional information SDS and EDTA did not inhibit enzyme activity Trametes polyzona
NaCl 1 M, 90% inhibition Trametes polyzona
Propanol 25%, 17% inhibition Trametes polyzona
Triton X 2%, 61% inhibition Trametes polyzona
Tween 80 2%, 51% inhibition Trametes polyzona

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
guaiacol pH and temperature not specified in the publication Trametes polyzona

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trametes polyzona
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag3+ 2.5 mM, activates to 115.94% of control Trametes polyzona
Al3+ 2.5 mM, activates to 109.42% of control Trametes polyzona
Cu2+ 2.5 mM, activates to 237.68% of control Trametes polyzona
Fe3+ 2.5 mM, activates to 114.49% of control Trametes polyzona
K+ 2.5 mM, activates to 117.39% of control Trametes polyzona
Mn2+ 2.5 mM, activates to 105.03% of control Trametes polyzona
Zn2+ 2.5 mM, activates to 102.89% of control Trametes polyzona

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
-
Trametes polyzona

Organism

Organism UniProt Comment Textmining
Trametes polyzona
-
-
-
Trametes polyzona MPS1-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Trametes polyzona

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guaiacol + O2
-
Trametes polyzona 6-methoxycyclohexa-2,4-dienone + H2O
-
?
guaiacol + O2
-
Trametes polyzona MPS1-3 6-methoxycyclohexa-2,4-dienone + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trametes polyzona

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 stability between 30°C and 60°C with a maximum at 50°C Trametes polyzona

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Trametes polyzona