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Literature summary for 1.10.3.2 extracted from
- Bacterial laccases promising biological green tools for industrial applications (2018), Cell. Mol. Life Sci., 75, 3569-3592.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | treating waste water containing synthetic dyes | Bacillus subtilis |
| additional information | pulp biobleaching | Bacillus tequilensis |
| paper production | biobleaching of eucalyptus kraft pulps results in a significant decrease in the kappa number and a significant increase in the brightness of the pulps | Streptomyces cyaneus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus pumilus |
| expression in Escherichia coli | Bacillus subtilis |
| expression in Escherichia coli | Bacillus vallismortis |
| expression in Escherichia coli | Streptomyces coelicolor |
| expression in Escherichia coli | Streptomyces cyaneus |
| expression in Escherichia coli or in Pichia pastoris | Bacillus licheniformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D500G | in Pichia pastoris 9.3folds higher expression than wild-type enzyme | Bacillus licheniformis |
| D501G | better stability and catalytic efficiency than wild-type enzyme | Bacillus amyloliquefaciens |
| E106F | mutation promotes both enzymatic activity and thermostability | Shigella dysenteriae |
| K316N | 11.4-fold higher expression level. High dimerization of phenolic and decolorization of industrial dyes | Bacillus licheniformis |
| Y108A | Tyr108 does form an integral part of the active site and affects enzyme kinetics | Streptomyces coelicolor |
| Y108F | Tyr108 does form an integral part of the active site and affects enzyme kinetics | Streptomyces coelicolor |
| Y229A | over 10fold increase in activity | Streptomyces coelicolor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus tequilensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | multicopper enzyme | Bacillus altitudinis |  |
| copper | multicopper enzyme | Bacillus amyloliquefaciens | |
| copper | multicopper enzyme | Bacillus cereus | |
| copper | multicopper enzyme | Bacillus licheniformis | |
| copper | multicopper enzyme | Bacillus pumilus | |
| copper | multicopper enzyme | Bacillus safensis | |
| copper | multicopper enzyme | Bacillus subtilis | |
| copper | multicopper enzyme | Bacillus tequilensis | |
| copper | multicopper enzyme | Bacillus vallismortis | |
| copper | multicopper enzyme | Halalkalibacterium halodurans | |
| copper | multicopper enzyme | Kitasatospora aureofaciens | |
| copper | multicopper enzyme | Lysinibacillus sphaericus | |
| copper | multicopper enzyme | Shigella dysenteriae | |
| copper | multicopper enzyme | Shouchella clausii | |
| copper | multicopper enzyme | Streptomyces bikiniensis | |
| copper | multicopper enzyme | Streptomyces coelicolor | |
| copper | multicopper enzyme | Streptomyces cyaneus | |
| copper | multicopper enzyme | Streptomyces griseus | |
| copper | multicopper enzyme | Streptomyces ipomoeae | |
| copper | multicopper enzyme | Streptomyces lavendulae | |
| copper | multicopper enzyme | Streptomyces lividans | |
| copper | multicopper enzyme | Streptomyces sviceus | |
| copper | multicopper enzyme | Streptomyces violaceusniger | |
| copper | multicopper enzyme | Streptomyces viridosporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58500 | - |
- |
Bacillus subtilis |
| 65000 | - |
- |
Bacillus pumilus |
| 69500 | - |
- |
Streptomyces cyaneus |
| 70000 | - |
- |
Bacillus vallismortis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus altitudinis | - |
- |
- |
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus cereus | - |
- |
- |
| Bacillus licheniformis | A0A6B9ET10 | - |
- |
| Bacillus pumilus | - |
- |
- |
| Bacillus pumilus | I3RYX9 | - |
- |
| Bacillus pumilus MK001 | I3RYX9 | - |
- |
| Bacillus pumilus W3 | - |
- |
- |
| Bacillus safensis | - |
- |
- |
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis | P07788 | - |
- |
| Bacillus subtilis | C6KEH7 | - |
- |
| Bacillus subtilis 168 | P07788 | - |
- |
| Bacillus subtilis cjp3 | - |
- |
- |
| Bacillus subtilis WD23 | C6KEH7 | - |
- |
| Bacillus tequilensis | - |
- |
- |
| Bacillus tequilensis SN4 | - |
- |
- |
| Bacillus vallismortis | S5MW00 | - |
- |
| Bacillus vallismortis fmb-103 | S5MW00 | - |
- |
| Halalkalibacterium halodurans | - |
- |
- |
| Kitasatospora aureofaciens | - |
- |
- |
| Lysinibacillus sphaericus | - |
- |
- |
| Shigella dysenteriae | - |
- |
- |
| Shouchella clausii | - |
- |
- |
| Streptomyces bikiniensis | - |
- |
- |
| Streptomyces coelicolor | Q9XAL8 | - |
- |
| Streptomyces coelicolor ATCC BAA-471 | Q9XAL8 | - |
- |
| Streptomyces cyaneus | F6L7B5 | - |
- |
| Streptomyces cyaneus CECT 3335 | F6L7B5 | - |
- |
| Streptomyces griseus | - |
- |
- |
| Streptomyces ipomoeae | A3QVH2 | - |
- |
| Streptomyces ipomoeae CECT 3341 | A3QVH2 | - |
- |
| Streptomyces lavendulae | Q8GB87 | - |
- |
| Streptomyces lividans | - |
- |
- |
| Streptomyces sviceus | - |
- |
- |
| Streptomyces violaceusniger | - |
- |
- |
| Streptomyces viridosporus | J9PBR2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endospore | spore-bound | Bacillus subtilis | - |
| endospore | - |
Bacillus licheniformis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus subtilis | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus vallismortis | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Streptomyces cyaneus | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus subtilis cjp3 | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus vallismortis fmb-103 | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Streptomyces cyaneus CECT 3335 | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus W3 | ? | - |
? | |
| 2,6-dimethylphenol + O2 | - |
Streptomyces cyaneus | ? | - |
? | |
| 2,6-dimethylphenol + O2 | - |
Streptomyces cyaneus CECT 3335 | ? | - |
? | |
| acetosyringone + O2 | - |
Bacillus vallismortis | ? | - |
? | |
| acetosyringone + O2 | - |
Bacillus vallismortis fmb-103 | ? | - |
? | |
| guaiacol + O2 | - |
Streptomyces cyaneus | 6-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
| guaiacol + O2 | - |
Streptomyces cyaneus CECT 3335 | 6-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
| Malachite Green + O2 | - |
Bacillus vallismortis | ? | - |
? | |
| Malachite Green + O2 | - |
Bacillus vallismortis fmb-103 | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus subtilis | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus vallismortis | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus subtilis cjp3 | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus vallismortis fmb-103 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Benzenediol:oxygen oxidoreductase | - |
Bacillus subtilis |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus pumilus |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus licheniformis |
| Benzenediol:oxygen oxidoreductase | - |
Halalkalibacterium halodurans |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus vallismortis |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus tequilensis |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus amyloliquefaciens |
| Benzenediol:oxygen oxidoreductase | - |
Lysinibacillus sphaericus |
| Benzenediol:oxygen oxidoreductase | - |
Shouchella clausii |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus altitudinis |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus safensis |
| Benzenediol:oxygen oxidoreductase | - |
Bacillus cereus |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces coelicolor |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces cyaneus |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces griseus |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces lavendulae |
| Benzenediol:oxygen oxidoreductase | - |
Kitasatospora aureofaciens |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces ipomoeae |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces sviceus |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces bikiniensis |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces violaceusniger |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces viridosporus |
| Benzenediol:oxygen oxidoreductase | - |
Streptomyces lividans |
| Benzenediol:oxygen oxidoreductase | - |
Shigella dysenteriae |
| CotA-laccase | - |
Bacillus subtilis |
| CotA-laccase | - |
Bacillus pumilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus pumilus |
| 80 | - |
- |
Bacillus subtilis |
| 80 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Streptomyces cyaneus |
| 85 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus vallismortis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | stable from 20°C-80°C | Bacillus subtilis |
| 50 | - |
6 h, 50% loss of activity | Bacillus pumilus |
| 60 | - |
1 h, 50% loss of activity | Streptomyces cyaneus |
| 70 | - |
10 h, more than 50% of activity | Bacillus vallismortis |
| 70 | - |
half-life: 100 min | Streptomyces lavendulae |
| 80 | - |
half-life: 112 min | Bacillus subtilis |
| 80 | - |
half-life: 2.5 h | Bacillus subtilis |
| 80 | - |
half-life: 1 h | Bacillus pumilus |
| 80 | - |
half-life: 1.14 h | Bacillus pumilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus pumilus |
| 4.8 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus vallismortis |
| 5 | - |
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Bacillus subtilis |
| 5.5 | - |
substrate: 2,6-dimethylphthalate | Streptomyces cyaneus |
| 7.4 | - |
substrate: syringaldazine | Bacillus vallismortis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at alkaline pH | Bacillus pumilus |
| 5.5 | - |
24 h, 40% of activity | Streptomyces cyaneus |
| 6.8 | - |
half-life: 6 months | Bacillus subtilis |
| 9 | - |
half-life: 15 days | Bacillus subtilis |
| 9 | - |
stable for 10 h | Bacillus subtilis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.72 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH and temperature not specified in the publication | Streptomyces cyaneus | |
| 5.17 | - |
guaiacol | pH and temperature not specified in the publication | Streptomyces cyaneus | |
| 157.86 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH and temperature not specified in the publication | Bacillus pumilus | |
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