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Literature summary for 1.10.3.2 extracted from

  • Ezike, T.C.; Ezugwu, A.L.; Udeh, J.O.; Eze, S.O.O.; Chilaka, F.C.
    Purification and characterisation of new laccase from Trametes polyzona WRF03 (2020), Biotechnol. Rep., 28, e00566 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, 26% loss of activity Trametes polyzona
Ca2+ 30 mM, 85% loss of activity Trametes polyzona
Co2+ 10 mM, 99% loss of activity Trametes polyzona
Cu2+ 10 mM, 23% loss of activity Trametes polyzona
EDTA 1 mM, 74.2% inhibition Trametes polyzona
Fe2+ 10 mM, 97% loss of activity Trametes polyzona
Hg2+ 10 mM, 80% loss of activity Trametes polyzona
L-cysteine 1 mM, 65.05% inhibition Trametes polyzona
Mg2+ 10 mM, 23% loss of activity Trametes polyzona
Mn2+ 50 mM, 100% loss of activity Trametes polyzona
Pb2+ 10 mM, 56% loss of activity Trametes polyzona
SDS 1 mM, 83.33% inhibition Trametes polyzona
Sodium azide 1 mM, 91.4% inhibition Trametes polyzona
Sodium cyanide 1 mM, 81.18% inhibition Trametes polyzona
Urea 45.69% inhibition Trametes polyzona
Zn2+ 50 mM, 97% loss of activity Trametes polyzona

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00866
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 25°C Trametes polyzona

Metals/Ions

Metals/Ions Comment Organism Structure
copper multi-copper containing oxidase Trametes polyzona
Mg2+ 50 mM, slight stimulation (105%) Trametes polyzona

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 SDS-PAGE Trametes polyzona

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10% (v/v), the enzyme retains more than 89.68% activity Trametes polyzona
acetonitrile 10% (v/v), the enzyme retains more than 93.38% activity Trametes polyzona
Ethanol 10% (v/v), the enzyme retains more than 92.55% activity. At 50% (v/v), the enzyme loses about 85% activity after 1 h Trametes polyzona
Methanol 10% (v/v), the enzyme retains more than 94% activity Trametes polyzona
propanol 10% (v/v), the enzyme retains more than 92.89% activity Trametes polyzona

Organism

Organism UniProt Comment Textmining
Trametes polyzona
-
-
-
Trametes polyzona WRF03
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trametes polyzona

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes polyzona WRF03 ?
-
 ?
Coomassie brilliant blue + O2 72.35% decolourisation efficiency Trametes polyzona ?
-
 ?
Coomassie brilliant blue + O2 72.35% decolourisation efficiency Trametes polyzona WRF03 ?
-
 ?
Erichrome black + O2 40.20% decolourisation efficiency Trametes polyzona ?
-
 ?
Erichrome black + O2 40.20% decolourisation efficiency Trametes polyzona WRF03 ?
-
 ?
Malachite Green + O2 57.84% decolourisation efficiency Trametes polyzona ?
-
 ?
Malachite Green + O2 57.84% decolourisation efficiency Trametes polyzona WRF03 ?
-
 ?
Methyl orange + O2 47.55% decolourisation efficiency Trametes polyzona ?
-
 ?
additional information low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %) Trametes polyzona ?
-
-
additional information low decolourisation efficiency towards Azure B (1.78 %) and methylene blue (0.38 %) Trametes polyzona WRF03 ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Trametes polyzona

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Trametes polyzona

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 95 25°C: about 50% of maximal activity, 95°C: about 40% of maximal activity Trametes polyzona

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, about 20% loss of activity Trametes polyzona
40 50 most stable at a temperature range of 40-50°C Trametes polyzona
50
-
 20 min, 25% loss of activity Trametes polyzona
60
-
 20 min, about 80% loss of activity Trametes polyzona
70
-
 5 min, 90% loss of activity Trametes polyzona

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Trametes polyzona

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 pH 3.5: about 70% of maximal activity, pH 5.0: about 70% of maximal activity Trametes polyzona

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 120 min, the enzyme retains about 82% activity Trametes polyzona
5.5 6.5 most stable within pH of 5.5-6.5 Trametes polyzona
6
-
 120 min, the enzyme retains about 100% activity Trametes polyzona
6.5
-
 120 min, the enzyme retains about 93% activity Trametes polyzona