Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Zhang, Y.; Dai, Z.; Zhang, S.; Yang, X.
    The catalytic properties of Thermus thermophilus SG0.5JP17-16 laccase were regulated by the conformational dynamics of pocket loop 6 (2021), Biochim. Biophys. Acta Gen. Subj., 1865, 129872 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
D394E mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 50%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 Thermus thermophilus
D394M mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 50%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 Thermus thermophilus
D394R mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 16%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 Thermus thermophilus
D396A mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme Thermus thermophilus
D396E mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme Thermus thermophilus
D396M mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.215
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394M Thermus thermophilus
0.243
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394R Thermus thermophilus
0.266
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394E Thermus thermophilus
0.325
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396A Thermus thermophilus
0.356
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396M Thermus thermophilus
0.382
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396E Thermus thermophilus
0.392
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396N Thermus thermophilus
0.407
-
 guaiacol pH 6.0, 90°C, wild-type enzyme Thermus thermophilus
0.439
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394N Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
copper multicopper oxidase Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus F6DF14
-
-
Thermus thermophilus SG0.5JP17-16 F6DF14
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Congo Red + O2 the enzyme decolorizes the azo dye Thermus thermophilus ?
-
 ?
Congo Red + O2 the enzyme decolorizes the azo dye Thermus thermophilus SG0.5JP17-16 ?
-
 ?
guaiacol + O2
-
 Thermus thermophilus 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
guaiacol + O2
-
 Thermus thermophilus SG0.5JP17-16 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
Reactive Black B + O2 the enzyme decolorizes the azo dye Thermus thermophilus ?
-
 ?
Reactive Black B + O2 the enzyme decolorizes the azo dye Thermus thermophilus SG0.5JP17-16 ?
-
 ?
Reactive Black WNN + O2 the enzyme decolorizes the azo dye Thermus thermophilus ?
-
 ?
Reactive Black WNN + O2 the enzyme decolorizes the azo dye Thermus thermophilus SG0.5JP17-16 ?
-
 ?
Remazol Brilliant Blue R + O2 the enzyme decolorizes the anthraquinone dye Thermus thermophilus ?
-
 ?
Remazol Brilliant Blue R + O2 the enzyme decolorizes the anthraquinone dye Thermus thermophilus SG0.5JP17-16 ?
-
 ?

Synonyms

Synonyms Comment Organism
lacTT
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 wild-type enzyme and mutant enzymes D394E, D394M and D394R Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 4 h, wild-type enzymed retains 80% of its activity. The thermal stability of D394R mutant decreases significantly, with the residual activity of 15% after 4 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394R Thermus thermophilus
1.7
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394M Thermus thermophilus
3.3
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394E Thermus thermophilus
6.7
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396N Thermus thermophilus
6.9
-
 guaiacol pH 6.0, 90°C, wild-type enzyme Thermus thermophilus
7.1
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396A Thermus thermophilus
7.3
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396M Thermus thermophilus
7.4
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394N Thermus thermophilus
7.4
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396E Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 wild-type enzyme Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.3
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394R Thermus thermophilus
7.9
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394M Thermus thermophilus
12.4
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394E Thermus thermophilus
16.8
-
 guaiacol pH 6.0, 90°C, mutant enzyme D394N Thermus thermophilus
16.9
-
 guaiacol pH 6.0, 90°C, wild-type enzyme Thermus thermophilus
17.1
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396N Thermus thermophilus
19.4
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396E Thermus thermophilus
20.5
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396M Thermus thermophilus
21.8
-
 guaiacol pH 6.0, 90°C, mutant enzyme D396A Thermus thermophilus