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Literature summary for 1.10.3.2 extracted from

  • Clement, R.; Wang, X.; Biaso, F.; Ilbert, M.; Mazurenko, I.; Lojou, E.
    Mutations in the coordination spheres of T1 Cu affect Cu2+-activation of the laccase from Thermus thermophilus (2021), Biochimie, 182, 228-237 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Protein Variants

Protein Variants Comment Organism
M455L mutation in T1 Cu site, incorporation of 3-4 copper atoms, similar to wild-type. Mutation results in an increase of 100 mV in the O2 reduction potential, while the enzymatic activity for ABTS oxidation is decreased Thermus thermophilus
M456A mutation in T1 Cu site, incorporation of 3-4 copper atoms, similar to wild-type Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M455L, 30°C, pH 5 Thermus thermophilus
1.58
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus
2.33
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus
2.94
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, 30°C, pH 5 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72HW2 bifunctional copper oxidase and laccase, cf. EC 1.16.3.4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2 i.e. ABTS Thermus thermophilus ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus
0.16
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M455L, 30°C, pH 5 Thermus thermophilus
0.25
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, 30°C, pH 5 Thermus thermophilus
0.69
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.065
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M456A, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus
0.085
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, 30°C, pH 5 Thermus thermophilus
0.437
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) wild-type, presence of 0.5 mM Cu2+, 30°C, pH 5 Thermus thermophilus
0.521
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) mutant M455L, 30°C, pH 5 Thermus thermophilus