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Literature summary for 1.10.3.2 extracted from
- Modifications of laccase activities of copper efflux oxidase, CueO by synergistic mutations in the first and second coordination spheres of the type I copper center (2013), Biochem. Biophys. Res. Commun., 431, 393-397 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D439A | 3.8-4.2 copper atoms per protein molecule, similar to wild-type, redox potential is 0.43 V compared to 0.36 for wild-type | Escherichia coli |
| D439A/M510L | 140fold and 44fold increases in the kcat values for ABTS and 2,5-diaminotoluene, redox potential is 0.39 V | Escherichia coli |
| D439A/M510Q | redox potential of the mutant s 0.21 V | Escherichia coli |
| D439A/P444A | 40fold increase in the ABTS-oxidizing activity, redox potential is 0.46 V | Escherichia coli |
| D439A/P444A/M510L | T1 copper in the triple mutant cannot be fully oxidized resulting in loss of enzymatic activities | Escherichia coli |
| D439A/P444A/M510Q | redox potential of the mutant was 0.26 V and high enzymatic activities are not attained | Escherichia coli |
| M510L | 3.8-4.2 copper atoms per protein molecule, similar to wild-type, redox potential is 0.40 V | Escherichia coli |
| M510Q | 3.8-4.2 copper atoms per protein molecule, similar to wild-type, redox potential is 0.23 V | Escherichia coli |
| P444A | 3.8-4.2 copper atoms per protein molecule, similar to wild-type, redox potential is 0.43 V | Escherichia coli |
| P444A/M510L | enzymatic activities similar to wild-type | Escherichia coli |
| P444A/M510Q | 3.4 copper atoms per protein molecule, similar to wild-type, redox potential is 0.21 V, no oxidizing activity of ABTS is observed | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | mutant D439A/M510L, pH 5.5, temperature not specified in the publication | Escherichia coli |  |
| 1.4 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | mutant P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 4.2 | - |
2,5-diaminotoluene | mutant M510L, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 5.5 | - |
2,5-diaminotoluene | mutant D439A/M510L, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 6.7 | - |
2,5-diaminotoluene | mutant D439A/P444A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 9.3 | - |
2,5-diaminotoluene | mutant P444A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 12 | - |
2,5-diaminotoluene | mutant D439A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 12 | - |
2,5-diaminotoluene | wild-type, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 14 | - |
2,5-diaminotoluene | mutant P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P36649 | bifunctional copper oxidase and laccase, cf. EC 1.16.3.4 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2 | i.e. ABTS | Escherichia coli | ? | - |
? | |
| 2,5-diaminotoluene + O2 | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CueO | - |
Escherichia coli |
| YacK | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | mutant P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 3 | 6 | 2,5-diaminotoluene | mutant M510L, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 3.2 | - |
2,5-diaminotoluene | mutant D439A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 11 | - |
2,5-diaminotoluene | mutant P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 16 | - |
2,5-diaminotoluene | mutant D439A/P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 19 | - |
2,5-diaminotoluene | wild-type, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 25 | - |
2,5-diaminotoluene | mutant P444A/M510Q, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 105 | - |
2,5-diaminotoluene | mutant P444A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 137 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | mutant D439A/M510L, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 233 | - |
2,5-diaminotoluene | mutant D439A/P444A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 366 | - |
2,5-diaminotoluene | mutant D439A, pH 5.5, temperature not specified in the publication | Escherichia coli | |
| 832 | - |
2,5-diaminotoluene | mutant D439A/M510L, pH 5.5, temperature not specified in the publication | Escherichia coli |
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