Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Zymomonas mobilis |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, crystal structure of oxidized preGFOR R30K/R31K, in complex with succinate (preGFOR(succ)) and with glycerol (PreGFOR(gll)), at 2.2 A and 2.05 A resolution, respectively, and of reduced preGFOR R30K/R31K, after incubation with glucose (preGFOR(Glu)) and with sorbitol (preGFOR(sorb)) at 2.5 A and 2.6 A resolution. In all four crystal structures, the signal peptide is disordered, implying a flexibility that may be important for its interaction with the translocation apparatus. The crystal structures show that the mature enzyme portion of preGFOR is identical to native GFOR, in structure and cofactor binding | Zymomonas mobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | translocated across the membrane in fully folded form. A twin-arginine motif in the signal peptide directs it to a Sec-independent pathway by which it is translocated, in fully folded form, into the periplasm where it functions to produce sorbitol for osmoprotection | Zymomonas mobilis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zymomonas mobilis | in periplasm the enzyme functions to produce sorbitol for osmoprotection | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | GFOR is synthesized in the cytoplasm with a 52-residue signal peptide, giving a precursor form, preGFOR, that is fully active and has its cofactor tightly bound | Zymomonas mobilis |
Purification (Comment) | Organism |
---|---|
- |
Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in periplasm the enzyme functions to produce sorbitol for osmoprotection | Zymomonas mobilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GFOR | - |
Zymomonas mobilis |
NADP(H)-dependent glucose-fructose oxidoreductase | - |
Zymomonas mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | tightly bound | Zymomonas mobilis | |
NADPH | tightly bound | Zymomonas mobilis |