Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Zymomonas mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanol | 16% inhibition at 2 M, 48% inhibition at 4.7 M | Zymomonas mobilis | |
sorbitol | 0.8 M, 27% inhibition | Zymomonas mobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
170000 | - |
gel filtration at pH 5.0 | Zymomonas mobilis |
300000 | - |
gel filtration at pH 7.0 | Zymomonas mobilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose + D-fructose | Zymomonas mobilis | enzyme is responsible for sorbitol production | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zymomonas mobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
- |
Zymomonas mobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Deoxy-D-glucose + D-fructose | at 6% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
D-Galactose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
D-glucose + D-fructose | - |
Zymomonas mobilis | D-glucono-1,5-lactone + D-sorbitol | - |
? | |
D-Glucose + D-fructose | enzyme is responsible for sorbitol production | Zymomonas mobilis | ? | - |
? | |
D-Glucose + D-xylulose | at 7% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
D-Glucose + dihydroxyacetone | at 6% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
D-Glucose + L-sorbose | at 0.5% of the activity relative to fructose | Zymomonas mobilis | ? | - |
? | |
D-Mannose + D-fructose | at 12% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
D-Xylose + D-fructose | at 8% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
DL-Glyceraldehyde + D-fructose | at 1.5% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? | |
L-Arabinose + D-fructose | at 3% of the activity relative to D-glucose | Zymomonas mobilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, at pH 5, SDS-PAGE | Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
sorbitol | - |
Zymomonas mobilis | |
0.8 | - |
gluconolactone | - |
Zymomonas mobilis | |
200 | - |
D-glucose | - |
Zymomonas mobilis | |
200 | - |
D-fructose | - |
Zymomonas mobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Zymomonas mobilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.1 | pH 5.0: about 35% of maximal activity, pH 7.1: about 30% of maximal activity | Zymomonas mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | tightly bound as hydrogen carrier | Zymomonas mobilis | |
NADPH | tightly bound as hydrogen carrier | Zymomonas mobilis |