BRENDA - Enzyme Database
show all sequences of 1.1.99.22

5-Keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in Gluconobacter species

Matsushita, K.; Fujii, Y.; Ano, Y.; Toyama, H.; Shinjoh, M.; Tomiyama, N.; Miyazaki, T.; Sugisawa, T.; Hoshino, T.; Adachi, O.; Appl. Environ. Microbiol. 69, 1959-1966 (2003)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
enzyme disruption mutant, oxidation activity against D-arabitol, D-sorbitol, D-mannitol, ribitol, meso-erythritol and glycerol are diminished
Gluconobacter oxydans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
cytoplasmic membrane
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Gluconobacter oxydans
production of 5-keto-D-gluconate is solely dependent on enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
identical with D-sorbitol dehydrogenase, EC1.1.99.21, and D-arabitol dehydrogenase, EC1.1.1.11
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + acceptor
-
654267
Gluconobacter oxydans
D-xylulose + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
654267
Gluconobacter oxydans
5-keto-D-gluconate + reduced acceptor
-
-
-
?
D-mannitol + acceptor
-
654267
Gluconobacter oxydans
D-fructose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
-
654267
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
glycerol + acceptor
-
654267
Gluconobacter oxydans
dihydroxyacetone + reduced acceptor
-
-
-
?
meso-erythritol + acceptor
-
654267
Gluconobacter oxydans
L-erythrulose + reduced acceptor
-
-
-
?
additional information
production of 5-keto-D-gluconate is solely dependent on enzyme
654267
Gluconobacter oxydans
?
-
-
-
-
ribitol + acceptor
-
654267
Gluconobacter oxydans
? + reduced acceptor
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter oxydans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
9
-
about 50% of activity at pH 5.0
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
2,6-dichlorophenolindophenol
acts in vitro as acceptor together with phenazine methosulfate
Gluconobacter oxydans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
enzyme disruption mutant, oxidation activity against D-arabitol, D-sorbitol, D-mannitol, ribitol, meso-erythritol and glycerol are diminished
Gluconobacter oxydans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
cytoplasmic membrane
Gluconobacter oxydans
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Gluconobacter oxydans
production of 5-keto-D-gluconate is solely dependent on enzyme
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabitol + acceptor
-
654267
Gluconobacter oxydans
D-xylulose + reduced acceptor
-
-
-
?
D-gluconate + acceptor
-
654267
Gluconobacter oxydans
5-keto-D-gluconate + reduced acceptor
-
-
-
?
D-mannitol + acceptor
-
654267
Gluconobacter oxydans
D-fructose + reduced acceptor
-
-
-
?
D-sorbitol + acceptor
-
654267
Gluconobacter oxydans
L-sorbose + reduced acceptor
-
-
-
?
glycerol + acceptor
-
654267
Gluconobacter oxydans
dihydroxyacetone + reduced acceptor
-
-
-
?
meso-erythritol + acceptor
-
654267
Gluconobacter oxydans
L-erythrulose + reduced acceptor
-
-
-
?
additional information
production of 5-keto-D-gluconate is solely dependent on enzyme
654267
Gluconobacter oxydans
?
-
-
-
-
ribitol + acceptor
-
654267
Gluconobacter oxydans
? + reduced acceptor
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Gluconobacter oxydans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
9
-
about 50% of activity at pH 5.0
Gluconobacter oxydans
Other publictions for EC 1.1.99.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740188
Ano
Membrane-bound glycerol dehydr ...
Gluconobacter thailandicus, Gluconobacter thailandicus NBRC 3258
Biosci. Biotechnol. Biochem.
81
411-418
2017
-
-
-
-
-
-
1
-
1
2
1
-
-
3
-
-
1
-
-
-
-
-
16
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
2
1
-
-
-
-
1
-
-
-
-
16
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721386
Hattori
High-temperature sorbose ferme ...
Gluconobacter frateurii, Gluconobacter frateurii CHM43, NBRC 101659
Appl. Microbiol. Biotechnol.
95
1531-1540
2012
-
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
6
-
1
1
-
-
1
-
-
-
-
1
1
-
-
-
721866
Habe
Disruption of the membrane-bou ...
Gluconobacter oxydans, Gluconobacter oxydans NBRC12528
Biosci. Biotechnol. Biochem.
74
1391-1395
2010
-
-
-
-
-
-
-
-
2
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
721867
Habe
Use of a Gluconobacter frateur ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
74
2330-2332
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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-
1
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-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
695781
Saichana
Screening of thermotolerant Gl ...
Gluconobacter sp., Gluconobacter sp. THE42
Appl. Environ. Microbiol.
75
4240-4247
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685690
Soemphol
Distinct physiological roles o ...
Gluconobacter frateurii, Gluconobacter frateurii THD32
Biosci. Biotechnol. Biochem.
72
842-850
2008
-
-
-
-
-
-
-
-
1
-
-
2
-
7
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
-
1
-
-
2
-
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-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
-
-
1
-
-
-
-
-
-
1
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
674959
Michizoe
Functionalization of the cytoc ...
Escherichia coli, Escherichia coli JM109
J. Biosci. Bioeng.
99
12-17
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
31
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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-
-
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-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654267
Matsushita
5-Keto-D-gluconate production ...
Gluconobacter oxydans
Appl. Environ. Microbiol.
69
1959-1966
2003
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287791
Ameyama
-
Solubilization, purification a ...
Gluconobacter oxydans, Gluconobacter oxydans IFO 3260
Agric. Biol. Chem.
49
1001-1010
1985
-
-
-
-
-
3
1
4
1
-
-
-
-
2
-
-
1
-
-
-
1
2
13
-
1
-
-
-
1
-
1
4
-
-
-
-
-
-
4
-
-
3
-
1
-
4
1
-
-
-
-
-
-
1
-
-
1
2
13
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-